The transcriptome of Populus in elevated CO2 reveals increased anthocyanin biosynthesis during delayed autumnal senescence
The transcriptome of Populus in elevated CO2 reveals increased anthocyanin biosynthesis during delayed autumnal senescence
The delay in autumnal senescence that has occurred in recent decades has been linked to rising temperatures. Here, we suggest that increasing atmospheric CO2 may partly account for delayed autumnal senescence and for the first time, through transcriptome analysis, identify gene expression changes associated with this delay.
Using a plantation of Populus × euramericana grown in elevated [CO2] (e[CO2]) with free-air CO2 enrichment (FACE) technology, we investigated the molecular and biochemical basis of this response. A Populus cDNA microarray was used to identify genes representing multiple biochemical pathways influenced by e[CO2] during senescence. Gene expression changes were confirmed through real-time quantitative PCR, and leaf biochemical assays.
Pathways for secondary metabolism and glycolysis were significantly up-regulated by e[CO2] during senescence, in particular, those related to anthocyanin biosynthesis. Expressed sequence tags (ESTs) representing the two most significantly up-regulated transcripts in e[CO2], LDOX (leucoanthocyanidin dioxgenase) and DFR (dihydroflavonol reductase), gave (e[CO2]/ambient CO2 (a[CO2])) expression ratios of 39.6 and 19.3, respectively.
We showed that in e[CO2] there was increased autumnal leaf sugar accumulation and up-regulation of genes determining anthocyanin biosynthesis which, we propose, prolongs leaf longevity during natural autumnal senescence.
anthocyanin biosynthesis, autumnal senescence, cdna microarray, elevated CO2, ldox, populus
415-428
Tallis, M.J.
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Lin, Y.
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Rogers, A.
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Zhang, J.
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Street, N.R.
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Karnosky, D.F.
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Miglietta, F.
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Calfapietra, C.
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De Angelis, P.
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April 2010
Tallis, M.J.
25cbd143-1f8c-491b-9eac-f1c632dfcfda
Lin, Y.
7086cb21-5f6b-48cc-add3-2efb33746dbf
Rogers, A.
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Zhang, J.
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Street, N.R.
de7bdc59-4af6-4e90-bf6d-5d3209b1a499
Karnosky, D.F.
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Miglietta, F.
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Calfapietra, C.
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De Angelis, P.
596ab4e4-9153-4350-bd61-0b891c6117fd
Tallis, M.J., Lin, Y., Rogers, A., Zhang, J., Street, N.R., Karnosky, D.F., Miglietta, F., Calfapietra, C., De Angelis, P. and Taylor, G.
(2010)
The transcriptome of Populus in elevated CO2 reveals increased anthocyanin biosynthesis during delayed autumnal senescence.
New Phytologist, 186 (2), .
(doi:10.1111/j.1469-8137.2010.03184.x).
(PMID:20202130)
Abstract
The delay in autumnal senescence that has occurred in recent decades has been linked to rising temperatures. Here, we suggest that increasing atmospheric CO2 may partly account for delayed autumnal senescence and for the first time, through transcriptome analysis, identify gene expression changes associated with this delay.
Using a plantation of Populus × euramericana grown in elevated [CO2] (e[CO2]) with free-air CO2 enrichment (FACE) technology, we investigated the molecular and biochemical basis of this response. A Populus cDNA microarray was used to identify genes representing multiple biochemical pathways influenced by e[CO2] during senescence. Gene expression changes were confirmed through real-time quantitative PCR, and leaf biochemical assays.
Pathways for secondary metabolism and glycolysis were significantly up-regulated by e[CO2] during senescence, in particular, those related to anthocyanin biosynthesis. Expressed sequence tags (ESTs) representing the two most significantly up-regulated transcripts in e[CO2], LDOX (leucoanthocyanidin dioxgenase) and DFR (dihydroflavonol reductase), gave (e[CO2]/ambient CO2 (a[CO2])) expression ratios of 39.6 and 19.3, respectively.
We showed that in e[CO2] there was increased autumnal leaf sugar accumulation and up-regulation of genes determining anthocyanin biosynthesis which, we propose, prolongs leaf longevity during natural autumnal senescence.
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e-pub ahead of print date: 23 February 2010
Published date: April 2010
Keywords:
anthocyanin biosynthesis, autumnal senescence, cdna microarray, elevated CO2, ldox, populus
Organisations:
Biological Sciences
Identifiers
Local EPrints ID: 145889
URI: http://eprints.soton.ac.uk/id/eprint/145889
ISSN: 0028-646X
PURE UUID: a1fb0ddc-e3a0-4286-a513-389923025b3c
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Date deposited: 20 Apr 2010 08:30
Last modified: 14 Mar 2024 00:52
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Contributors
Author:
M.J. Tallis
Author:
Y. Lin
Author:
A. Rogers
Author:
J. Zhang
Author:
N.R. Street
Author:
D.F. Karnosky
Author:
F. Miglietta
Author:
C. Calfapietra
Author:
P. De Angelis
Author:
G. Taylor
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