Thiamine biosynthesis in Escherichia coli: Identification of the intermediate and by-product derived from tyrosine
Thiamine biosynthesis in Escherichia coli: Identification of the intermediate and by-product derived from tyrosine
In anaerobic organisms such as E. coli the tyrosine lyase ThiH is essential for the biosynthesis of the thiazole moiety of the vitamin thiamine. ThiH is a member of the radical AdoMet family. The products formed by cleavage of tyrosine in vitro have been identified and suggest a radical-mediated cleavage resulting in p-cresol and dehydroglycine which is hydrolyzed to glyoxylate.
thiazole, synthase, bacillus-subtilis, moiety, carrier protein, radicals, thiamine, complex, reconstitution, enzyme mechanisms, p-hydroxyphenylacetate decarboxylase, biosynthesis, in-vitro, protein superfamily
9223-9226
Kriek, M.
e01ddb12-38a7-4733-9e13-c0e5d9670cca
Martins, F.M.R.
ba52d38a-d72c-41ec-a86d-d6aaacf0f1e4
Challand, M.R
e887b78b-7545-4c64-846a-ade3be4e6b99
Croft, A.
504b1978-b29a-4d4f-8781-edbc5fb486a8
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
30 October 2007
Kriek, M.
e01ddb12-38a7-4733-9e13-c0e5d9670cca
Martins, F.M.R.
ba52d38a-d72c-41ec-a86d-d6aaacf0f1e4
Challand, M.R
e887b78b-7545-4c64-846a-ade3be4e6b99
Croft, A.
504b1978-b29a-4d4f-8781-edbc5fb486a8
Roach, P.L.
ca94060c-4443-482b-af3e-979243488ba9
Kriek, M., Martins, F.M.R., Challand, M.R, Croft, A. and Roach, P.L.
(2007)
Thiamine biosynthesis in Escherichia coli: Identification of the intermediate and by-product derived from tyrosine.
Angewandte Chemie International Edition, 46 (48), .
(doi:10.1002/anie.200702554).
Abstract
In anaerobic organisms such as E. coli the tyrosine lyase ThiH is essential for the biosynthesis of the thiazole moiety of the vitamin thiamine. ThiH is a member of the radical AdoMet family. The products formed by cleavage of tyrosine in vitro have been identified and suggest a radical-mediated cleavage resulting in p-cresol and dehydroglycine which is hydrolyzed to glyoxylate.
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Published date: 30 October 2007
Keywords:
thiazole, synthase, bacillus-subtilis, moiety, carrier protein, radicals, thiamine, complex, reconstitution, enzyme mechanisms, p-hydroxyphenylacetate decarboxylase, biosynthesis, in-vitro, protein superfamily
Organisations:
Chemistry
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Local EPrints ID: 146169
URI: http://eprints.soton.ac.uk/id/eprint/146169
ISSN: 1433-7851
PURE UUID: 9fed7c59-18dc-46b2-848b-0fa4f00c8e84
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Date deposited: 03 Jun 2010 11:00
Last modified: 14 Mar 2024 00:54
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Contributors
Author:
M. Kriek
Author:
F.M.R. Martins
Author:
M.R Challand
Author:
A. Croft
Author:
P.L. Roach
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