Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy
Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy
The penetration of light into optically thick samples containing the G-protein-coupled receptor rhodopsin is studied by numerical finite-element simulations and double-quantum solid-state NMR experiments. Illumination with white light leads to the generation of the active bathorhodopsin photostate in the outer layer of the sample but generates a large amount of the side product, isorhodopsin, in the sample interior. The overall yield of bathorhodopsin is improved by using monochromatic 420 nm illumination and by mixing the sample with transparent glass beads. The implications of these findings on the interpretation of previously published rhodopsin NMR data are discussed.
6133-6140
Concistre, Maria
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Gansmuller, Axel
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McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Johannessen, Ole G.
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Marin Montesinos, Ildefonso
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Bovee-Geurts, Petra H.M.
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Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
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Levitt, Malcolm H.
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6 May 2009
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Gansmuller, Axel
239d731b-d3cb-4c2f-8c9e-ddb1e195c73f
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
Marin Montesinos, Ildefonso
3a84d29e-130d-4983-9834-03bc0beeb86f
Bovee-Geurts, Petra H.M.
66d6099e-73b5-4f84-ae7a-2b61d874e4e9
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Concistre, Maria, Gansmuller, Axel, McLean, Neville, Johannessen, Ole G., Marin Montesinos, Ildefonso, Bovee-Geurts, Petra H.M., Brown, Richard C.D., DeGrip, Willem J. and Levitt, Malcolm H.
(2009)
Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy.
Journal of the American Chemical Society, 131 (17), .
(doi:10.1021/ja809878c).
Abstract
The penetration of light into optically thick samples containing the G-protein-coupled receptor rhodopsin is studied by numerical finite-element simulations and double-quantum solid-state NMR experiments. Illumination with white light leads to the generation of the active bathorhodopsin photostate in the outer layer of the sample but generates a large amount of the side product, isorhodopsin, in the sample interior. The overall yield of bathorhodopsin is improved by using monochromatic 420 nm illumination and by mixing the sample with transparent glass beads. The implications of these findings on the interpretation of previously published rhodopsin NMR data are discussed.
Other
ja809878c
- Version of Record
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e-pub ahead of print date: 8 April 2009
Published date: 6 May 2009
Identifiers
Local EPrints ID: 146421
URI: http://eprints.soton.ac.uk/id/eprint/146421
ISSN: 0002-7863
PURE UUID: 2322edd4-7682-4e9e-aed5-65ab578dd24a
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Date deposited: 21 Apr 2010 13:15
Last modified: 14 Mar 2024 02:54
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Contributors
Author:
Maria Concistre
Author:
Axel Gansmuller
Author:
Neville McLean
Author:
Ole G. Johannessen
Author:
Ildefonso Marin Montesinos
Author:
Petra H.M. Bovee-Geurts
Author:
Willem J. DeGrip
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