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Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy

Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy
Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy
The penetration of light into optically thick samples containing the G-protein-coupled receptor rhodopsin is studied by numerical finite-element simulations and double-quantum solid-state NMR experiments. Illumination with white light leads to the generation of the active bathorhodopsin photostate in the outer layer of the sample but generates a large amount of the side product, isorhodopsin, in the sample interior. The overall yield of bathorhodopsin is improved by using monochromatic 420 nm illumination and by mixing the sample with transparent glass beads. The implications of these findings on the interpretation of previously published rhodopsin NMR data are discussed.
0002-7863
6133-6140
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Gansmuller, Axel
239d731b-d3cb-4c2f-8c9e-ddb1e195c73f
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
Marin Montesinos, Ildefonso
3a84d29e-130d-4983-9834-03bc0beeb86f
Bovee-Geurts, Petra H.M.
66d6099e-73b5-4f84-ae7a-2b61d874e4e9
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Gansmuller, Axel
239d731b-d3cb-4c2f-8c9e-ddb1e195c73f
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
Marin Montesinos, Ildefonso
3a84d29e-130d-4983-9834-03bc0beeb86f
Bovee-Geurts, Petra H.M.
66d6099e-73b5-4f84-ae7a-2b61d874e4e9
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3

Concistre, Maria, Gansmuller, Axel, McLean, Neville, Johannessen, Ole G., Marin Montesinos, Ildefonso, Bovee-Geurts, Petra H.M., Brown, Richard C.D., DeGrip, Willem J. and Levitt, Malcolm H. (2009) Light penetration and photoisomerization in rhodopsin studied by numerical simulations and double-quantum solid-state NMR spectroscopy. Journal of the American Chemical Society, 131 (17), 6133-6140. (doi:10.1021/ja809878c).

Record type: Article

Abstract

The penetration of light into optically thick samples containing the G-protein-coupled receptor rhodopsin is studied by numerical finite-element simulations and double-quantum solid-state NMR experiments. Illumination with white light leads to the generation of the active bathorhodopsin photostate in the outer layer of the sample but generates a large amount of the side product, isorhodopsin, in the sample interior. The overall yield of bathorhodopsin is improved by using monochromatic 420 nm illumination and by mixing the sample with transparent glass beads. The implications of these findings on the interpretation of previously published rhodopsin NMR data are discussed.

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e-pub ahead of print date: 8 April 2009
Published date: 6 May 2009

Identifiers

Local EPrints ID: 146421
URI: http://eprints.soton.ac.uk/id/eprint/146421
ISSN: 0002-7863
PURE UUID: 2322edd4-7682-4e9e-aed5-65ab578dd24a
ORCID for Richard C.D. Brown: ORCID iD orcid.org/0000-0003-0156-7087
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

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Date deposited: 21 Apr 2010 13:15
Last modified: 20 Jul 2019 01:16

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Contributors

Author: Maria Concistre
Author: Axel Gansmuller
Author: Neville McLean
Author: Ole G. Johannessen
Author: Ildefonso Marin Montesinos
Author: Petra H.M. Bovee-Geurts
Author: Willem J. DeGrip

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