Click chemistry with DNA
Click chemistry with DNA
The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyne–azide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency. It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. In this critical review we describe some of the pioneering work that has been carried out in this area (78 references).
1388-1405
El-Sagheer, Afaf H.
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Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
April 2010
El-Sagheer, Afaf H.
0bf56d90-78ee-4772-add8-453ccacc39be
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
El-Sagheer, Afaf H. and Brown, Tom
(2010)
Click chemistry with DNA.
Chemical Society Reviews, 39 (4), .
(doi:10.1039/b901971p).
Abstract
The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyne–azide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency. It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. In this critical review we describe some of the pioneering work that has been carried out in this area (78 references).
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Click_Chemistry_with_DNA_Chem_Soc_Rev_2010.pdf
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Published date: April 2010
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Local EPrints ID: 146661
URI: http://eprints.soton.ac.uk/id/eprint/146661
ISSN: 0306-0012
PURE UUID: 8be7330b-bc82-4f18-9364-790c023b150b
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Date deposited: 22 Apr 2010 08:26
Last modified: 14 Mar 2024 00:56
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Author:
Afaf H. El-Sagheer
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