The use of an electroactive marker as a SERS label in an E-melting mutation discrimination assay
The use of an electroactive marker as a SERS label in an E-melting mutation discrimination assay
In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting (E-melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22-mer oligonucleotides labelled with a modified anthraquinone at the 3-end. Discrimination between the wild type, 1653C/T (point mutation) and F508 (triple deletion) is demonstrated
DNA hydridization, SERS, electrochemical melting curve, anthraquinone label, Base pair mismatches
2190-2197
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Richardson, James
51db9f73-a136-48af-8722-21d46906cf40
Gaied, Nouha Ben
06ac4241-0292-4224-a100-81aeab66f098
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
23 September 2009
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Richardson, James
51db9f73-a136-48af-8722-21d46906cf40
Gaied, Nouha Ben
06ac4241-0292-4224-a100-81aeab66f098
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Mahajan, Sumeet, Richardson, James, Gaied, Nouha Ben, Zhao, Zhengyun, Brown, Tom and Bartlett, Philip N.
(2009)
The use of an electroactive marker as a SERS label in an E-melting mutation discrimination assay.
Electroanalysis, 21 (20), .
(doi:10.1002/elan.200904681).
Abstract
In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting (E-melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22-mer oligonucleotides labelled with a modified anthraquinone at the 3-end. Discrimination between the wild type, 1653C/T (point mutation) and F508 (triple deletion) is demonstrated
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Published date: 23 September 2009
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Times Cited: 7
Keywords:
DNA hydridization, SERS, electrochemical melting curve, anthraquinone label, Base pair mismatches
Identifiers
Local EPrints ID: 146669
URI: http://eprints.soton.ac.uk/id/eprint/146669
ISSN: 1040-0397
PURE UUID: 825360f9-728b-4cd3-aca1-6ce61a181843
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Date deposited: 22 Apr 2010 08:30
Last modified: 14 Mar 2024 02:52
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Contributors
Author:
James Richardson
Author:
Nouha Ben Gaied
Author:
Zhengyun Zhao
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