The University of Southampton
University of Southampton Institutional Repository

Potent triple helix stabilization by 5?,3?-modified triplex-forming oligonucleotides

Potent triple helix stabilization by 5?,3?-modified triplex-forming oligonucleotides
Potent triple helix stabilization by 5?,3?-modified triplex-forming oligonucleotides
Anthraquinone and pyrene analogues attached to the 3 and/or 5 termini of triplex-forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid-phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 °C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 °C at pH 7 even though they do not contain any triplexstabilizing base analogues. These studies have implications for the design of triplex-forming oligonucleotides for use in biology and nanotechnology.
anthraquinone, dna recognition, dna, pyrene, triple helix
1439-4227
1839-1851
Ben Gaied, Nouha
e97c4e6d-2eaf-4c2b-8b42-f82ac35671ff
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Ben Gaied, Nouha
e97c4e6d-2eaf-4c2b-8b42-f82ac35671ff
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Ben Gaied, Nouha, Zhao, Zhengyun, Gerrard, Simon R., Fox, Keith R. and Brown, Tom (2009) Potent triple helix stabilization by 5?,3?-modified triplex-forming oligonucleotides. ChemBioChem, 10 (11), 1839-1851. (doi:10.1002/cbic.200900232). (PMID:19554592)

Record type: Article

Abstract

Anthraquinone and pyrene analogues attached to the 3 and/or 5 termini of triplex-forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid-phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 °C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 °C at pH 7 even though they do not contain any triplexstabilizing base analogues. These studies have implications for the design of triplex-forming oligonucleotides for use in biology and nanotechnology.

Full text not available from this repository.

More information

Published date: 20 July 2009
Keywords: anthraquinone, dna recognition, dna, pyrene, triple helix

Identifiers

Local EPrints ID: 146671
URI: https://eprints.soton.ac.uk/id/eprint/146671
ISSN: 1439-4227
PURE UUID: 7a444f59-7586-433b-9fd6-74abf7e8ac96
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 22 Apr 2010 08:33
Last modified: 06 Jun 2018 13:16

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×