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Closed nanoconstructs assembled by step-by-step ss-DNA coupling assisted by phospholipid membranes

Closed nanoconstructs assembled by step-by-step ss-DNA coupling assisted by phospholipid membranes
Closed nanoconstructs assembled by step-by-step ss-DNA coupling assisted by phospholipid membranes
We report experimental evidence of the construction of closed pseudo-hexagonal DNA nanostructures grafted onto lipid membranes (supported planar bilayers or liposomes).

The anchoring agent is a cholesteryl tetraethylenglycol-18-mer oligonucleotide, which recruits the complementary sequences added in a sequential fashion. A closed rather than an open nanostructure was chosen as a test for possible future applications in nanoelectronics and for the fact that a more rigid and defined structure can offer many advantages in terms of addressability and spatial control.

The so-built hybrid soft nanomaterial has never been reported previously and merges the unique features offered by DNA building blocks in nanotechnology, to the characteristics of amphiphilic self-assembly, in terms of responsiveness and further hierarchical aggregation in functional arrays of nano-units. The liposomes decorated with DNA pseudo-hexagons are structurally stable for weeks and can thus be further exploited or specifically addressed.

The structural features of the final nanoobjects are independent on the sequence of preparation, i.e. step-wise on the membrane or addition of preformed hexagons, up to a threshold of density on the surface or vesicle number density.
1744-683X
1639-1645
Baldelli Bombelli, Francesca
92c757ea-b6ce-424f-a568-d318d6798f01
Betti, Francesca
01c7237b-2331-4508-9cce-230c21b0169e
Gambinossi, Filippo
8f600237-8a29-4e52-bbe0-5dea1e0447b4
Caminati, Gabriella
841c7c0f-fff9-4b17-8e41-c35cc76c502a
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Baglioni, Piero
695e2f89-0770-4f24-866c-2ef50f274d9d
Berti, Debora
fc2212b2-4a40-4302-8258-e32bf5a3fd25
Baldelli Bombelli, Francesca
92c757ea-b6ce-424f-a568-d318d6798f01
Betti, Francesca
01c7237b-2331-4508-9cce-230c21b0169e
Gambinossi, Filippo
8f600237-8a29-4e52-bbe0-5dea1e0447b4
Caminati, Gabriella
841c7c0f-fff9-4b17-8e41-c35cc76c502a
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Baglioni, Piero
695e2f89-0770-4f24-866c-2ef50f274d9d
Berti, Debora
fc2212b2-4a40-4302-8258-e32bf5a3fd25

Baldelli Bombelli, Francesca, Betti, Francesca, Gambinossi, Filippo, Caminati, Gabriella, Brown, Tom, Baglioni, Piero and Berti, Debora (2009) Closed nanoconstructs assembled by step-by-step ss-DNA coupling assisted by phospholipid membranes. Soft Matter, 5 (8), 1639-1645. (doi:10.1039/b816716h).

Record type: Article

Abstract

We report experimental evidence of the construction of closed pseudo-hexagonal DNA nanostructures grafted onto lipid membranes (supported planar bilayers or liposomes).

The anchoring agent is a cholesteryl tetraethylenglycol-18-mer oligonucleotide, which recruits the complementary sequences added in a sequential fashion. A closed rather than an open nanostructure was chosen as a test for possible future applications in nanoelectronics and for the fact that a more rigid and defined structure can offer many advantages in terms of addressability and spatial control.

The so-built hybrid soft nanomaterial has never been reported previously and merges the unique features offered by DNA building blocks in nanotechnology, to the characteristics of amphiphilic self-assembly, in terms of responsiveness and further hierarchical aggregation in functional arrays of nano-units. The liposomes decorated with DNA pseudo-hexagons are structurally stable for weeks and can thus be further exploited or specifically addressed.

The structural features of the final nanoobjects are independent on the sequence of preparation, i.e. step-wise on the membrane or addition of preformed hexagons, up to a threshold of density on the surface or vesicle number density.

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Published date: 2009

Identifiers

Local EPrints ID: 146677
URI: https://eprints.soton.ac.uk/id/eprint/146677
ISSN: 1744-683X
PURE UUID: 40aab9f5-9e68-4fd1-8d99-4531baed27cc

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Date deposited: 22 Apr 2010 08:42
Last modified: 17 Jul 2019 00:04

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Contributors

Author: Francesca Baldelli Bombelli
Author: Francesca Betti
Author: Filippo Gambinossi
Author: Gabriella Caminati
Author: Tom Brown
Author: Piero Baglioni
Author: Debora Berti

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