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Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage

Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage
Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage
DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3?-AZT and 5?-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplicons and clones in two different sequence contexts revealed the presence of a single thymidine at the ligation site. The remarkable ability of thermostable polymerases to reproducibly copy DNA templates containing such an unnatural backbone opens up intriguing possibilities in gene synthesis, genetic analysis, biology, and nanotechnology.
0002-7863
3958-3964
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

El-Sagheer, Afaf H. and Brown, Tom (2009) Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage Journal of the American Chemical Society, 131, (11), pp. 3958-3964. (doi:10.1021/ja8065896).

Record type: Article

Abstract

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3?-AZT and 5?-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplicons and clones in two different sequence contexts revealed the presence of a single thymidine at the ligation site. The remarkable ability of thermostable polymerases to reproducibly copy DNA templates containing such an unnatural backbone opens up intriguing possibilities in gene synthesis, genetic analysis, biology, and nanotechnology.

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e-pub ahead of print date: 27 February 2009
Published date: 25 March 2009
Organisations: Organic Chemistry: SCF

Identifiers

Local EPrints ID: 146679
URI: http://eprints.soton.ac.uk/id/eprint/146679
ISSN: 0002-7863
PURE UUID: a5fe5f64-e4c2-4143-92ca-30fe8efbd4bb

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Date deposited: 22 Apr 2010 08:48
Last modified: 18 Jul 2017 23:04

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Contributors

Author: Afaf H. El-Sagheer
Author: Tom Brown

University divisions

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