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Rapid typing of STRs in the human genome by HyBeacon® melting

Rapid typing of STRs in the human genome by HyBeacon® melting
Rapid typing of STRs in the human genome by HyBeacon® melting
A new method based on DNA melting has been developed for the rapid analysis of STRs in the human genome. The system is based on homogeneous PCR followed by fluorescence melting analysis and utilises a HyBeacon® probe combined with a PCR primer-blocker oligonucleotide. The use of blockers of different length permits identification of the full range of common D16S539 repeats enabling detection of 99.8% of known alleles. The interrogation of STRs can be carried out on standard genetic analysis platforms and could be applied to other loci to form the basis of a bespoke high-throughput system for use in forensic analysis, particularly as fluorescent genetic analysis platforms are now available for high-resolution melting. This methodology may be suitable for rapid forensic DNA analysis at the point-of-arrest or in a custody suite where it is important to identify an individual from a small group of suspects/detainees.
1477-0520
4553-4559
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
French, David J.
019c0355-a863-48a4-a21b-f534d2da96b4
Howard, Rebecca L.
a23d7650-7143-485a-bba7-d417cb3cdc0c
McDowell, David G.
0dece0e9-770f-4db4-835d-28655aa86856
Debenham, Paul G.
ae162b2d-e340-48a9-ac98-849d3466438f
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
French, David J.
019c0355-a863-48a4-a21b-f534d2da96b4
Howard, Rebecca L.
a23d7650-7143-485a-bba7-d417cb3cdc0c
McDowell, David G.
0dece0e9-770f-4db4-835d-28655aa86856
Debenham, Paul G.
ae162b2d-e340-48a9-ac98-849d3466438f
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472

Gale, Nittaya, French, David J., Howard, Rebecca L., McDowell, David G., Debenham, Paul G. and Brown, Tom (2008) Rapid typing of STRs in the human genome by HyBeacon® melting. Organic & Biomolecular Chemistry, 6 (24), 4553-4559. (doi:10.1039/b813431f).

Record type: Article

Abstract

A new method based on DNA melting has been developed for the rapid analysis of STRs in the human genome. The system is based on homogeneous PCR followed by fluorescence melting analysis and utilises a HyBeacon® probe combined with a PCR primer-blocker oligonucleotide. The use of blockers of different length permits identification of the full range of common D16S539 repeats enabling detection of 99.8% of known alleles. The interrogation of STRs can be carried out on standard genetic analysis platforms and could be applied to other loci to form the basis of a bespoke high-throughput system for use in forensic analysis, particularly as fluorescent genetic analysis platforms are now available for high-resolution melting. This methodology may be suitable for rapid forensic DNA analysis at the point-of-arrest or in a custody suite where it is important to identify an individual from a small group of suspects/detainees.

Full text not available from this repository.

More information

Published date: 30 October 2008

Identifiers

Local EPrints ID: 146693
URI: http://eprints.soton.ac.uk/id/eprint/146693
ISSN: 1477-0520
PURE UUID: c9370e01-56a4-40dc-adbf-dfc74445d92c

Catalogue record

Date deposited: 22 Apr 2010 09:00
Last modified: 17 Jul 2019 00:04

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