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Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer
Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer
A synthetic library of ca. 1013 single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3?. A single aptamer, which showed similar affinities (KD not, vert, similar 10?8 M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep
aptamer, 14-3-3, TSE, CJD, affinity, protein–DNA interaction
0045-2068
215-219
Stevenson, R.
9b5b6be1-a1e4-4576-83d6-93a0c69484e2
Baxter, H.
22bf593e-0514-4703-a47b-a3fb67f50cbc
Aitken, A.
cdabaac2-822e-40b1-b158-61a2cfc1cef9
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
BAXTER, R.
447b6fb4-538e-4166-ac97-fad8a1a1cd02
Stevenson, R.
9b5b6be1-a1e4-4576-83d6-93a0c69484e2
Baxter, H.
22bf593e-0514-4703-a47b-a3fb67f50cbc
Aitken, A.
cdabaac2-822e-40b1-b158-61a2cfc1cef9
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
BAXTER, R.
447b6fb4-538e-4166-ac97-fad8a1a1cd02

Stevenson, R., Baxter, H., Aitken, A., Brown, Tom and BAXTER, R. (2008) Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer. Bioorganic Chemistry, 36 (5), 215-219. (doi:10.1016/j.bioorg.2007.12.005).

Record type: Article

Abstract

A synthetic library of ca. 1013 single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3?. A single aptamer, which showed similar affinities (KD not, vert, similar 10?8 M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep

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More information

Published date: October 2008
Keywords: aptamer, 14-3-3, TSE, CJD, affinity, protein–DNA interaction

Identifiers

Local EPrints ID: 146695
URI: http://eprints.soton.ac.uk/id/eprint/146695
ISSN: 0045-2068
PURE UUID: 914dae31-a7a3-4828-9185-c40e58415568

Catalogue record

Date deposited: 22 Apr 2010 09:11
Last modified: 17 Jul 2019 00:04

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