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Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening

Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening
Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening
Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR SGM Plus system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1h.

short tandem repeats (strss), fluorescent probe, hybeacon, melting curve analysis, real-time pcr
333-339
French, D.
9949f4cb-a9bb-44c9-abc6-1af3b86b3e11
Howard, R.
a9c7f87f-c5d4-4b32-a86a-a3264a516abe
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
McDowell, D.
fa93a31b-7969-4308-a6c0-b65022eb99ec
Debenham, P.
342baf36-6c3c-4898-be79-794ebaa8ff67
French, D.
9949f4cb-a9bb-44c9-abc6-1af3b86b3e11
Howard, R.
a9c7f87f-c5d4-4b32-a86a-a3264a516abe
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
McDowell, D.
fa93a31b-7969-4308-a6c0-b65022eb99ec
Debenham, P.
342baf36-6c3c-4898-be79-794ebaa8ff67

French, D., Howard, R., Gale, Nittaya, Brown, Tom, McDowell, D. and Debenham, P. (2008) Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening. Forensic Science International: Genetics, 2 (4), 333-339. (doi:10.1016/j.fsigen.2008.04.007).

Record type: Article

Abstract

Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR SGM Plus system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1h.

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More information

Published date: September 2008
Keywords: short tandem repeats (strss), fluorescent probe, hybeacon, melting curve analysis, real-time pcr

Identifiers

Local EPrints ID: 146699
URI: http://eprints.soton.ac.uk/id/eprint/146699
PURE UUID: a1fb935a-d077-4f15-93f3-890c46a8ce30

Catalogue record

Date deposited: 22 Apr 2010 09:05
Last modified: 17 Jul 2019 00:04

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