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Phospholipid membranes decorated by cholesterol-based oligonucleotides as soft hybrid nanostructures

Phospholipid membranes decorated by cholesterol-based oligonucleotides as soft hybrid nanostructures
Phospholipid membranes decorated by cholesterol-based oligonucleotides as soft hybrid nanostructures
DNA monomers and oligomers are currently showing great promise as building blocks for supramolecular arrays that can self-assemble in a fashion preprogrammed by the base pairing code. The design and build-up of hybrid DNA/amphiphilic self-assemblies can expand the range of possible architectures and enhance the selectivity toward a well-specified geometry. We report on the self-assembly properties in aqueous solution of a cholesteryl?tetraethylenglycol single stranded 18-mer oligonucleotide (ON1TEG-Chol) and on its spontaneous insertion in fluid phospholipid membranes. Up to 500 units of these lipophilic ss-oligonucleotides can be incorporated in the outer leaflet of 350 Å radius POPC vesicle. The insertion and hybridization with the complementary oligonucleotide are monitored through light scattering as an increase of hydrodynamic thickness, which is interpreted in terms of average distance between anchoring sites. The conformation of the ss-oligonucleotidic portion is strongly dependent on surface coverage, passing from a quasi-random coil to a more rigid configuration, as concentration increases. Interestingly, conformational details affect in a straightforward fashion the hybridization kinetics. Liposomes with single- and double-strand decorations remain stable within the experimental time window (about one week). The structure represents an example of successful and stable amphiphile/DNA supramolecular hybrid, where a DNA guest is held in a membrane by hydrophobic interactions. The lipophilic oligonucleotide under investigation is therefore a suitable building block that can effectively serve as a hydrophobic anchor in the fluid bilayer to assemble supramolecular constructs based on the DNA digital code.
1520-5207
10942-10952
Banchelli, Martina
3957a944-1e5e-46c4-8741-38d8e65c02ee
Betti, Francesca
01c7237b-2331-4508-9cce-230c21b0169e
Berti, Debora
fc2212b2-4a40-4302-8258-e32bf5a3fd25
Caminati, Gabriella
841c7c0f-fff9-4b17-8e41-c35cc76c502a
Bombelli, Francesca Baldelli
6afe2a22-054d-4461-8b34-33e261563808
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Wilhelmsson, L. Marcus
bcf4f67a-198c-4c5e-80b8-a78f743509d4
Nordén, Bengt
64d4009e-7456-4490-ac87-8d587334c7e0
Baglioni, P.
2c48bba3-10f0-4e62-9f76-335b07b53f89
Banchelli, Martina
3957a944-1e5e-46c4-8741-38d8e65c02ee
Betti, Francesca
01c7237b-2331-4508-9cce-230c21b0169e
Berti, Debora
fc2212b2-4a40-4302-8258-e32bf5a3fd25
Caminati, Gabriella
841c7c0f-fff9-4b17-8e41-c35cc76c502a
Bombelli, Francesca Baldelli
6afe2a22-054d-4461-8b34-33e261563808
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Wilhelmsson, L. Marcus
bcf4f67a-198c-4c5e-80b8-a78f743509d4
Nordén, Bengt
64d4009e-7456-4490-ac87-8d587334c7e0
Baglioni, P.
2c48bba3-10f0-4e62-9f76-335b07b53f89

Banchelli, Martina, Betti, Francesca, Berti, Debora, Caminati, Gabriella, Bombelli, Francesca Baldelli, Brown, Tom, Wilhelmsson, L. Marcus, Nordén, Bengt and Baglioni, P. (2008) Phospholipid membranes decorated by cholesterol-based oligonucleotides as soft hybrid nanostructures. The Journal of Physical Chemistry B, 112 (35), 10942-10952. (doi:10.1021/jp802415t).

Record type: Article

Abstract

DNA monomers and oligomers are currently showing great promise as building blocks for supramolecular arrays that can self-assemble in a fashion preprogrammed by the base pairing code. The design and build-up of hybrid DNA/amphiphilic self-assemblies can expand the range of possible architectures and enhance the selectivity toward a well-specified geometry. We report on the self-assembly properties in aqueous solution of a cholesteryl?tetraethylenglycol single stranded 18-mer oligonucleotide (ON1TEG-Chol) and on its spontaneous insertion in fluid phospholipid membranes. Up to 500 units of these lipophilic ss-oligonucleotides can be incorporated in the outer leaflet of 350 Å radius POPC vesicle. The insertion and hybridization with the complementary oligonucleotide are monitored through light scattering as an increase of hydrodynamic thickness, which is interpreted in terms of average distance between anchoring sites. The conformation of the ss-oligonucleotidic portion is strongly dependent on surface coverage, passing from a quasi-random coil to a more rigid configuration, as concentration increases. Interestingly, conformational details affect in a straightforward fashion the hybridization kinetics. Liposomes with single- and double-strand decorations remain stable within the experimental time window (about one week). The structure represents an example of successful and stable amphiphile/DNA supramolecular hybrid, where a DNA guest is held in a membrane by hydrophobic interactions. The lipophilic oligonucleotide under investigation is therefore a suitable building block that can effectively serve as a hydrophobic anchor in the fluid bilayer to assemble supramolecular constructs based on the DNA digital code.

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Published date: 2008

Identifiers

Local EPrints ID: 146707
URI: https://eprints.soton.ac.uk/id/eprint/146707
ISSN: 1520-5207
PURE UUID: c509f478-3621-4c2f-a979-8cd0110b4395

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Date deposited: 22 Apr 2010 09:02
Last modified: 17 Jul 2019 00:04

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Contributors

Author: Martina Banchelli
Author: Francesca Betti
Author: Debora Berti
Author: Gabriella Caminati
Author: Francesca Baldelli Bombelli
Author: Tom Brown
Author: L. Marcus Wilhelmsson
Author: Bengt Nordén
Author: P. Baglioni

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