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Rapid and efficient DNA strand cross-linking by click chemistry

Rapid and efficient DNA strand cross-linking by click chemistry
Rapid and efficient DNA strand cross-linking by click chemistry
Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 °C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.
click chemistry, cross-linking, cuacc reaction, DNA
1439-4227
1280-1285
Kočalka, Petr
58bbd62e-4359-4e87-a980-cb835233dafb
El-Sagheer, Afaf H.
0bf56d90-78ee-4772-add8-453ccacc39be
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kočalka, Petr
58bbd62e-4359-4e87-a980-cb835233dafb
El-Sagheer, Afaf H.
0bf56d90-78ee-4772-add8-453ccacc39be
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Kočalka, Petr, El-Sagheer, Afaf H. and Brown, Tom (2008) Rapid and efficient DNA strand cross-linking by click chemistry. ChemBioChem, 9 (8), 1280-1285. (doi:10.1002/cbic.200800006). (PMID:18418819)

Record type: Article

Abstract

Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 °C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.

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More information

Published date: 23 May 2008
Keywords: click chemistry, cross-linking, cuacc reaction, DNA
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Identifiers

Local EPrints ID: 146709
URI: http://eprints.soton.ac.uk/id/eprint/146709
DOI: doi:10.1002/cbic.200800006
ISSN: 1439-4227
PURE UUID: 625b1d9d-c69f-47ca-99e4-cc0aabee547b

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Date deposited: 22 Apr 2010 09:07
Last modified: 14 Mar 2024 00:56

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Contributors

Author: Petr Kočalka
Author: Afaf H. El-Sagheer
Author: Tom Brown

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