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Controlled release of Chol-TEG-DNA from Nano- and micropatterned SU-8 surfaces by a spreading lipid film

Controlled release of Chol-TEG-DNA from Nano- and micropatterned SU-8 surfaces by a spreading lipid film
Controlled release of Chol-TEG-DNA from Nano- and micropatterned SU-8 surfaces by a spreading lipid film
We report the controlled release of immobilized cholesteryl-tetraethyleneglycol-DNA (chol-DNA) from micropatterned SU-8 surfaces by a spreading lipid film. The release of chol-DNA is rapid and on the order of the spreading rate of the lipid film ? = 1?3 ?m2/s (105 molecules of DNA per second). The lipid film serves as a poor solvent for the DNA adduct, which upon contact redistributes into the aqueous phase. Thus, the release of DNA is accompanied by a change in surface hydrophobicity. The method can be used for creating arbitrary concentration profiles of DNA in solution over time or to dynamically change surface properties on demand in, for example, micro- and nanofluidic devices. Examples of DNA release from spiral, comb, meander, and triangular as well as from nanoscale SU-8 lanes are shown.
1530-6984
227
Erkan, Yavuz
d2492f0d-2afd-40aa-8bb3-587ee2908acf
Halma, Kiona
4adc6632-07e3-4e2e-8b3d-d7f7f3aebabf
Czolkos, Ilja
04418544-9c17-4993-9779-ac7ed9c3a6c6
Jesorka, Aldo
2c99fa24-a8da-42fa-8847-fc5a689818e3
Dommersnes, Paul
1a15f47a-cf87-4d9f-ad02-b1f15c526700
Kumar, Ravindra
dfd53d15-f740-4a18-90ad-efa6f2c5e488
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Orwar, Owe
507d5098-e27d-4d04-ba76-a8173bdbf7ea
Erkan, Yavuz
d2492f0d-2afd-40aa-8bb3-587ee2908acf
Halma, Kiona
4adc6632-07e3-4e2e-8b3d-d7f7f3aebabf
Czolkos, Ilja
04418544-9c17-4993-9779-ac7ed9c3a6c6
Jesorka, Aldo
2c99fa24-a8da-42fa-8847-fc5a689818e3
Dommersnes, Paul
1a15f47a-cf87-4d9f-ad02-b1f15c526700
Kumar, Ravindra
dfd53d15-f740-4a18-90ad-efa6f2c5e488
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Orwar, Owe
507d5098-e27d-4d04-ba76-a8173bdbf7ea

Erkan, Yavuz, Halma, Kiona, Czolkos, Ilja, Jesorka, Aldo, Dommersnes, Paul, Kumar, Ravindra, Brown, Tom and Orwar, Owe (2008) Controlled release of Chol-TEG-DNA from Nano- and micropatterned SU-8 surfaces by a spreading lipid film. Nano Letters, 8 (1), 227. (doi:10.1021/nl0725087).

Record type: Article

Abstract

We report the controlled release of immobilized cholesteryl-tetraethyleneglycol-DNA (chol-DNA) from micropatterned SU-8 surfaces by a spreading lipid film. The release of chol-DNA is rapid and on the order of the spreading rate of the lipid film ? = 1?3 ?m2/s (105 molecules of DNA per second). The lipid film serves as a poor solvent for the DNA adduct, which upon contact redistributes into the aqueous phase. Thus, the release of DNA is accompanied by a change in surface hydrophobicity. The method can be used for creating arbitrary concentration profiles of DNA in solution over time or to dynamically change surface properties on demand in, for example, micro- and nanofluidic devices. Examples of DNA release from spiral, comb, meander, and triangular as well as from nanoscale SU-8 lanes are shown.

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Published date: January 2008

Identifiers

Local EPrints ID: 146915
URI: http://eprints.soton.ac.uk/id/eprint/146915
ISSN: 1530-6984
PURE UUID: 08e7a951-6b81-49ae-a041-7d307d5ea558

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Date deposited: 22 Apr 2010 15:09
Last modified: 17 Jul 2019 00:04

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Contributors

Author: Yavuz Erkan
Author: Kiona Halma
Author: Ilja Czolkos
Author: Aldo Jesorka
Author: Paul Dommersnes
Author: Ravindra Kumar
Author: Tom Brown
Author: Owe Orwar

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