The University of Southampton
University of Southampton Institutional Repository

End-capped HyBeacon probes for the analysis of human genetic polymorphisms related to warfarin metabolism

End-capped HyBeacon probes for the analysis of human genetic polymorphisms related to warfarin metabolism
End-capped HyBeacon probes for the analysis of human genetic polymorphisms related to warfarin metabolism
HyBeacon probes have been used to characterise SNPs in the CYP2C9 and VKORC1 genes associated with variations in the efficiency of warfarin metabolism. PCR amplification of genomic DNA and probe target melting analysis provided a robust and reliable method to differentiate polymorphic sequences at these loci.

Probes capped with 5-trimethoxystilbene were found to exhibit larger fluorescence differences between hybridised and dissociated states than uncapped probes, generating melting peaks of considerably improved height. 3-Pyrene modifications enhanced probe signal-to-noise and melting peak heights with the additional benefit of acting as PCR stoppers, and 5,3-doubly capped probes gave the best analytical results.
1477-0520
Ben Gaied, Nouha
e97c4e6d-2eaf-4c2b-8b42-f82ac35671ff
Richardson, James A.
7039cf0c-37d4-42be-adad-bce68cb19c88
Singleton, Daniel G.
d05cfb21-47bf-463a-8bd5-ae112cdc5773
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
French, David
08ea7ade-bc16-4181-875e-e79ae8eb8138
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Ben Gaied, Nouha
e97c4e6d-2eaf-4c2b-8b42-f82ac35671ff
Richardson, James A.
7039cf0c-37d4-42be-adad-bce68cb19c88
Singleton, Daniel G.
d05cfb21-47bf-463a-8bd5-ae112cdc5773
Zhao, Zhengyun
fdc7f812-c529-4680-8fe2-fdb0e6fc134e
French, David
08ea7ade-bc16-4181-875e-e79ae8eb8138
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Ben Gaied, Nouha, Richardson, James A., Singleton, Daniel G., Zhao, Zhengyun, French, David and Brown, Tom (2010) End-capped HyBeacon probes for the analysis of human genetic polymorphisms related to warfarin metabolism. Organic & Biomolecular Chemistry. (doi:10.1039/C001177K).

Record type: Article

Abstract

HyBeacon probes have been used to characterise SNPs in the CYP2C9 and VKORC1 genes associated with variations in the efficiency of warfarin metabolism. PCR amplification of genomic DNA and probe target melting analysis provided a robust and reliable method to differentiate polymorphic sequences at these loci.

Probes capped with 5-trimethoxystilbene were found to exhibit larger fluorescence differences between hybridised and dissociated states than uncapped probes, generating melting peaks of considerably improved height. 3-Pyrene modifications enhanced probe signal-to-noise and melting peak heights with the additional benefit of acting as PCR stoppers, and 5,3-doubly capped probes gave the best analytical results.

Full text not available from this repository.

More information

Published date: 2010

Identifiers

Local EPrints ID: 146927
URI: http://eprints.soton.ac.uk/id/eprint/146927
ISSN: 1477-0520
PURE UUID: ba6dd095-db98-4550-9557-0034b0ea88c6

Catalogue record

Date deposited: 22 Apr 2010 15:28
Last modified: 17 Jul 2019 00:04

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×