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Whole-blood culture is a valid low-cost method to measure monocytic cytokines - a comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Whole-blood culture is a valid low-cost method to measure monocytic cytokines - a comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes
Whole-blood culture is a valid low-cost method to measure monocytic cytokines - a comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes
Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19–40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L. acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r = 0.57, P < 0.001 versus r = 0.33, P = 0.01 for IL-6 and r = 0.43, P < 0.001 versus r = 0.30, P = 0.02 for TNF-?. Adjustment for a preceding 8-week dietary fatty acid-intervention did not change any of the associations. Based on measurements at three time-points 8 weeks apart the intra-individual variation was ? 50% smaller than the inter-individual variation (P < 0.05) in most whole-blood cytokine responses and LPS-stimulated IL-6 from PBMC. We conclude that whole-blood cultures are well-suited low-cost proxy-measures of monocytic cytokine production. Moreover, large inter-individual variation in cytokine production was demonstrated whereas the individual responses in whole-blood were reproducible even over long time-periods.
monocyte, mononuclear cell, whole-blood culture, inflammatory cytokines
0022-1759
95 -101
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Damsgaard, Camilla T.
0e446962-c7c7-4437-bdbf-c6ddd9153a42
Lauritzen, Lotte
455b1303-d03f-4303-b746-3839d084ddcc
Kjær, Tanja M.R.
fe9d7265-5939-4426-8fa2-a3101324a8d6
Frøkiær, Hanne
0dae1e17-c35b-4697-9e85-059ed50f3880
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Damsgaard, Camilla T.
0e446962-c7c7-4437-bdbf-c6ddd9153a42
Lauritzen, Lotte
455b1303-d03f-4303-b746-3839d084ddcc
Kjær, Tanja M.R.
fe9d7265-5939-4426-8fa2-a3101324a8d6
Frøkiær, Hanne
0dae1e17-c35b-4697-9e85-059ed50f3880

Calder, Philip C., Damsgaard, Camilla T., Lauritzen, Lotte, Kjær, Tanja M.R. and Frøkiær, Hanne (2009) Whole-blood culture is a valid low-cost method to measure monocytic cytokines - a comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes. Journal of Immunological Methods, 340, 95 -101. (doi:10.1016/j.jim.2008.10.005).

Record type: Article

Abstract

Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19–40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L. acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r = 0.57, P < 0.001 versus r = 0.33, P = 0.01 for IL-6 and r = 0.43, P < 0.001 versus r = 0.30, P = 0.02 for TNF-?. Adjustment for a preceding 8-week dietary fatty acid-intervention did not change any of the associations. Based on measurements at three time-points 8 weeks apart the intra-individual variation was ? 50% smaller than the inter-individual variation (P < 0.05) in most whole-blood cytokine responses and LPS-stimulated IL-6 from PBMC. We conclude that whole-blood cultures are well-suited low-cost proxy-measures of monocytic cytokine production. Moreover, large inter-individual variation in cytokine production was demonstrated whereas the individual responses in whole-blood were reproducible even over long time-periods.

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More information

Published date: 30 January 2009
Keywords: monocyte, mononuclear cell, whole-blood culture, inflammatory cytokines

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Local EPrints ID: 147179
URI: https://eprints.soton.ac.uk/id/eprint/147179
ISSN: 0022-1759
PURE UUID: e3425a7f-c3e9-470c-9d42-9f6b74cb23b8

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Date deposited: 23 Apr 2010 11:08
Last modified: 17 Jul 2019 00:04

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