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Effect of increasing concentration of ammonium acetate as an additive in supercritical fluid chromatography using CO2–methanol mobile phase

Effect of increasing concentration of ammonium acetate as an additive in supercritical fluid chromatography using CO2–methanol mobile phase
Effect of increasing concentration of ammonium acetate as an additive in supercritical fluid chromatography using CO2–methanol mobile phase
The effects of increasing concentrations of ammonium acetate additive in supercritical fluid chromatography were studied on silica, 2-ethyl-pyridine and endcapped 2-ethyl-pyridine stationary phases. The study involved the addition of increasing concentrations of the ammonium acetate either in the mobile phase modifier (methanol) or in the sample solvent. The effects of ammonium acetate on retention and peak shape of the analytes were evaluated. Compounds that exhibited satisfactory chromatographic behaviour in the absence of the additive were virtually unaffected by its presence in the mobile phase or sample solvent. Nevertheless, compounds that exhibited late elution and strongly tailing peak shapes when pure methanol was used showed dramatically improved chromatographic behaviour in the presence of the additive. Shorter retention was observed not only when the modifier was introduced in the mobile phase but also when it was in the sample solvent.

supercritical fluid chromatography, additives, ammonium acetate
6441-6450
Cazenave-Gassiot, Amaury
f53d5438-9fad-4fd6-98f9-3e289205dcd7
Boughtflower, Robert
46c96e9d-9e08-42a5-b18f-3e6b1813433c
Caldwell, Jeffrey
fe40bcd8-452b-4909-9179-dd81ce665ecc
Hitzel, Laure
c6dd9747-b0ea-4164-b6d9-8ec258f8c9c4
Holyoak, Claire
1b7406bf-831f-49c6-a013-f3d83a9b94d2
Lane, Stephen
d752df3b-872b-4fc9-b684-36354cd752af
Oakley, Paul
52d23360-9ae2-49a2-a81b-7c1958216315
Pullen, Frank
dcaefc39-4e98-4b57-b214-77789b6efb80
Richardson, Stefan
c746499f-a882-42b3-b813-01f95087048e
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Cazenave-Gassiot, Amaury
f53d5438-9fad-4fd6-98f9-3e289205dcd7
Boughtflower, Robert
46c96e9d-9e08-42a5-b18f-3e6b1813433c
Caldwell, Jeffrey
fe40bcd8-452b-4909-9179-dd81ce665ecc
Hitzel, Laure
c6dd9747-b0ea-4164-b6d9-8ec258f8c9c4
Holyoak, Claire
1b7406bf-831f-49c6-a013-f3d83a9b94d2
Lane, Stephen
d752df3b-872b-4fc9-b684-36354cd752af
Oakley, Paul
52d23360-9ae2-49a2-a81b-7c1958216315
Pullen, Frank
dcaefc39-4e98-4b57-b214-77789b6efb80
Richardson, Stefan
c746499f-a882-42b3-b813-01f95087048e
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea

Cazenave-Gassiot, Amaury, Boughtflower, Robert, Caldwell, Jeffrey, Hitzel, Laure, Holyoak, Claire, Lane, Stephen, Oakley, Paul, Pullen, Frank, Richardson, Stefan and Langley, G. John (2009) Effect of increasing concentration of ammonium acetate as an additive in supercritical fluid chromatography using CO2–methanol mobile phase. Journal of Chromatography A, 1216 (36), 6441-6450. (doi:10.1016/j.chroma.2009.07.022).

Record type: Article

Abstract

The effects of increasing concentrations of ammonium acetate additive in supercritical fluid chromatography were studied on silica, 2-ethyl-pyridine and endcapped 2-ethyl-pyridine stationary phases. The study involved the addition of increasing concentrations of the ammonium acetate either in the mobile phase modifier (methanol) or in the sample solvent. The effects of ammonium acetate on retention and peak shape of the analytes were evaluated. Compounds that exhibited satisfactory chromatographic behaviour in the absence of the additive were virtually unaffected by its presence in the mobile phase or sample solvent. Nevertheless, compounds that exhibited late elution and strongly tailing peak shapes when pure methanol was used showed dramatically improved chromatographic behaviour in the presence of the additive. Shorter retention was observed not only when the modifier was introduced in the mobile phase but also when it was in the sample solvent.

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More information

Published date: 4 September 2009
Keywords: supercritical fluid chromatography, additives, ammonium acetate

Identifiers

Local EPrints ID: 147637
URI: https://eprints.soton.ac.uk/id/eprint/147637
PURE UUID: 7ba4ea9f-f7e0-4468-9f6c-34583601547e
ORCID for G. John Langley: ORCID iD orcid.org/0000-0002-8323-7235

Catalogue record

Date deposited: 26 Apr 2010 10:20
Last modified: 06 Jun 2018 13:11

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Contributors

Author: Amaury Cazenave-Gassiot
Author: Robert Boughtflower
Author: Jeffrey Caldwell
Author: Laure Hitzel
Author: Claire Holyoak
Author: Stephen Lane
Author: Paul Oakley
Author: Frank Pullen
Author: Stefan Richardson
Author: G. John Langley ORCID iD

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