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Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

Anderson, Keith, Cooper, Jonathan M., Haswell, Stephen J., Marshall, Damian, Yin, Huabing and Zhang, Xunli (2010) Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes Analyst, 135, pp. 1282-1287. (doi:10.1039/c0an00031k).

Record type: Article

Abstract

A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 M and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of -naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 M.

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Published date: May 2010

Identifiers

Local EPrints ID: 147651
URI: http://eprints.soton.ac.uk/id/eprint/147651
ISSN: 0003-2654
PURE UUID: 889a7b2a-f81c-4b15-89cb-06d2985a24c3

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Date deposited: 26 Apr 2010 10:22
Last modified: 15 Sep 2017 16:34

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Contributors

Author: Keith Anderson
Author: Jonathan M. Cooper
Author: Stephen J. Haswell
Author: Damian Marshall
Author: Huabing Yin
Author: Xunli Zhang

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