Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes
Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes
A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 M and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of -naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 M.
1282-1287
Anderson, Keith
0404b53f-ee7f-453e-b4f2-f83e9a72ea9d
Cooper, Jonathan M.
c8f72162-161e-4187-a2a1-05ccbf03af5a
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
Marshall, Damian
5d7a846b-3c5a-4ae8-95e8-88695ea0f273
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
May 2010
Anderson, Keith
0404b53f-ee7f-453e-b4f2-f83e9a72ea9d
Cooper, Jonathan M.
c8f72162-161e-4187-a2a1-05ccbf03af5a
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
Marshall, Damian
5d7a846b-3c5a-4ae8-95e8-88695ea0f273
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Anderson, Keith, Cooper, Jonathan M., Haswell, Stephen J., Marshall, Damian, Yin, Huabing and Zhang, Xunli
(2010)
Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes.
Analyst, 135, .
(doi:10.1039/c0an00031k).
Abstract
A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 M and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of -naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 M.
Text
Hepatocytes_Analyst_2010_v135p1282-7.pdf
- Version of Record
More information
Published date: May 2010
Identifiers
Local EPrints ID: 147651
URI: http://eprints.soton.ac.uk/id/eprint/147651
ISSN: 0003-2654
PURE UUID: 889a7b2a-f81c-4b15-89cb-06d2985a24c3
Catalogue record
Date deposited: 26 Apr 2010 10:22
Last modified: 14 Mar 2024 02:52
Export record
Altmetrics
Contributors
Author:
Keith Anderson
Author:
Jonathan M. Cooper
Author:
Stephen J. Haswell
Author:
Damian Marshall
Author:
Huabing Yin
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
