Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes


Anderson, Keith, Cooper, Jonathan M., Haswell, Stephen J., Marshall, Damian, Yin, Huabing and Zhang, Xunli (2010) Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes Analyst, 135, pp. 1282-1287. (doi:10.1039/c0an00031k).

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Description/Abstract

A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 M and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of -naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 M.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1039/c0an00031k
ISSNs: 0003-2654 (print)
Related URLs:
Subjects: T Technology > T Technology (General)
ePrint ID: 147651
Date :
Date Event
May 2010Published
Date Deposited: 26 Apr 2010 10:22
Last Modified: 18 Apr 2017 14:36
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/147651

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