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The polyunsaturated fatty acid composition of hepatic and plasma lipids differ by both sex and dietary fat intake in rats

The polyunsaturated fatty acid composition of hepatic and plasma lipids differ by both sex and dietary fat intake in rats
The polyunsaturated fatty acid composition of hepatic and plasma lipids differ by both sex and dietary fat intake in rats
In rats and humans, females have higher liver and/or plasma docosahexaenoic acid (DHA) content than males. We hypothesized that the effect of variation in total fat or essential fatty acid intakes on liver and plasma fatty acid composition would differ between sexes. Rats were fed a low-fat soybean oil (LFS), high-fat soybean oil (HFS), or high-fat linseed oil (HFL) diet for 20 d. There were significant sex differences in LFS rats in proportions of (n-3) and (n-6) fatty acids in plasma and liver contingent on lipid class. Significant diet x sex interactions were observed for eicosapentaenoic acid (EPA), DHA, and arachidonic acid (AA) status. HFL females had a higher proportion of EPA in plasma and liver phosphatidylcholine (PC), DHA in liver triacylglycerol (TAG), and AA in plasma PC than HFS and LFS females. These findings show that the effect of varying dietary fat intake on (n-3) and (n-6) long-chain PUFA (LCPUFA) status is modified by sex. Liver phospholipid and TAG fatty acid product:substrate ratios suggested greater 6 desaturase (6D) activity in females than in males. The HFL diet induced higher 6D mRNA expression compared with the LFS or HFS diets and HFL females had 10% higher expression of 6D mRNA than HFL males. Together, these findings show that sex is an important determinant of the effect of variations in fat and fatty acid intake on LCPUFA status, which may have implications for recommendations for fat and fatty acid intake in humans.
0022-3166
245-250
Childs, Caroline E.
ea17ccc1-2eac-4f67-96c7-a0c4d9dfd9c5
Romeu-Nadd, Meritxell
39379930-ae8e-4a44-8c08-36fc749688ef
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Childs, Caroline E.
ea17ccc1-2eac-4f67-96c7-a0c4d9dfd9c5
Romeu-Nadd, Meritxell
39379930-ae8e-4a44-8c08-36fc749688ef
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Childs, Caroline E., Romeu-Nadd, Meritxell, Burdge, Graham C. and Calder, Philip C. (2009) The polyunsaturated fatty acid composition of hepatic and plasma lipids differ by both sex and dietary fat intake in rats. Journal of Nutrition, 140 (2), 245-250. (doi:10.3945/jn.109.115691).

Record type: Article

Abstract

In rats and humans, females have higher liver and/or plasma docosahexaenoic acid (DHA) content than males. We hypothesized that the effect of variation in total fat or essential fatty acid intakes on liver and plasma fatty acid composition would differ between sexes. Rats were fed a low-fat soybean oil (LFS), high-fat soybean oil (HFS), or high-fat linseed oil (HFL) diet for 20 d. There were significant sex differences in LFS rats in proportions of (n-3) and (n-6) fatty acids in plasma and liver contingent on lipid class. Significant diet x sex interactions were observed for eicosapentaenoic acid (EPA), DHA, and arachidonic acid (AA) status. HFL females had a higher proportion of EPA in plasma and liver phosphatidylcholine (PC), DHA in liver triacylglycerol (TAG), and AA in plasma PC than HFS and LFS females. These findings show that the effect of varying dietary fat intake on (n-3) and (n-6) long-chain PUFA (LCPUFA) status is modified by sex. Liver phospholipid and TAG fatty acid product:substrate ratios suggested greater 6 desaturase (6D) activity in females than in males. The HFL diet induced higher 6D mRNA expression compared with the LFS or HFS diets and HFL females had 10% higher expression of 6D mRNA than HFL males. Together, these findings show that sex is an important determinant of the effect of variations in fat and fatty acid intake on LCPUFA status, which may have implications for recommendations for fat and fatty acid intake in humans.

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Published date: 9 December 2009
Organisations: Faculty of Medicine, Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 148189
URI: https://eprints.soton.ac.uk/id/eprint/148189
ISSN: 0022-3166
PURE UUID: caa0093a-7124-47e6-b832-bad12b1dcca0
ORCID for Caroline E. Childs: ORCID iD orcid.org/0000-0001-6832-224X
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

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Date deposited: 27 Apr 2010 11:57
Last modified: 06 Jun 2018 13:11

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Author: Meritxell Romeu-Nadd

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