The University of Southampton
University of Southampton Institutional Repository

Optimisation of polymer scaffolds for retinal pigment epithelium (RPE) cell transplantation.

Optimisation of polymer scaffolds for retinal pigment epithelium (RPE) cell transplantation.
Optimisation of polymer scaffolds for retinal pigment epithelium (RPE) cell transplantation.
Aim: to evaluate a variety of copolymers as suitable scaffolds to facilitate retinal pigment epithelium (RPE) transplantation.

Methods: five blends of poly(L-lactic acid) (PLLA) with poly(D, L-lactic-glycolic acid) (PLGA) were manufactured by a solid-liquid phase separation technique. The blends were 10:90, 25:75, 50:50, 75:25, 90:10 (PLLA:PLGA). All blend ratios were validated by nuclear magnetic resonance spectroscopy. Samples of polymer blends were coated with laminin. Coated and uncoated blends were seeded with a human RPE cell line. Cell attachment, viability and retention of phenotype were assessed.

Results: as the lactide unit content increased pore size generally became smaller. The 25:75 PLLA:PLGA blend was the most porous (44%) and thinnest (134?) scaffold produced. ARPE-19 cells survived with minimal cell death and maintained their normal phenotype for up to four weeks.. Cell density was maintained with only one of the fabricated ratios (25% PLLA:75% PLGA). There was a consistent decrease in apoptotic cell death with time on laminin coated samples of this blend. A decrease in polymer thickness concomitant with an increase in porosity characteristic of degradation was observed with all polymer blends.

Conclusions: this study demonstrates that a 25:75 copolymer blend of PLLA:PLGA is a potentially useful scaffold for ocular cell transplantation
0007-1161
563-568
Thomson, Heather Anne Jane
7db0d6dd-4e3c-47b0-a281-9a04c0b70252
Treharne, Andrew John
a158bb09-9d41-4b3a-ae02-ccbd76dd9417
Walker, Paul
9235b363-f0a5-46ff-99c6-22de755d7ed5
Grossel, Martin Christopher
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew John
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Thomson, Heather Anne Jane
7db0d6dd-4e3c-47b0-a281-9a04c0b70252
Treharne, Andrew John
a158bb09-9d41-4b3a-ae02-ccbd76dd9417
Walker, Paul
9235b363-f0a5-46ff-99c6-22de755d7ed5
Grossel, Martin Christopher
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew John
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514

Thomson, Heather Anne Jane, Treharne, Andrew John, Walker, Paul, Grossel, Martin Christopher and Lotery, Andrew John (2011) Optimisation of polymer scaffolds for retinal pigment epithelium (RPE) cell transplantation. British Journal of Ophthalmology, 95 (4), 563-568. (doi:10.1136/bjo.2009.166728).

Record type: Article

Abstract

Aim: to evaluate a variety of copolymers as suitable scaffolds to facilitate retinal pigment epithelium (RPE) transplantation.

Methods: five blends of poly(L-lactic acid) (PLLA) with poly(D, L-lactic-glycolic acid) (PLGA) were manufactured by a solid-liquid phase separation technique. The blends were 10:90, 25:75, 50:50, 75:25, 90:10 (PLLA:PLGA). All blend ratios were validated by nuclear magnetic resonance spectroscopy. Samples of polymer blends were coated with laminin. Coated and uncoated blends were seeded with a human RPE cell line. Cell attachment, viability and retention of phenotype were assessed.

Results: as the lactide unit content increased pore size generally became smaller. The 25:75 PLLA:PLGA blend was the most porous (44%) and thinnest (134?) scaffold produced. ARPE-19 cells survived with minimal cell death and maintained their normal phenotype for up to four weeks.. Cell density was maintained with only one of the fabricated ratios (25% PLLA:75% PLGA). There was a consistent decrease in apoptotic cell death with time on laminin coated samples of this blend. A decrease in polymer thickness concomitant with an increase in porosity characteristic of degradation was observed with all polymer blends.

Conclusions: this study demonstrates that a 25:75 copolymer blend of PLLA:PLGA is a potentially useful scaffold for ocular cell transplantation

This record has no associated files available for download.

More information

Published date: 3 April 2011

Identifiers

Local EPrints ID: 148215
URI: http://eprints.soton.ac.uk/id/eprint/148215
ISSN: 0007-1161
PURE UUID: 53e69a8d-9f3c-4669-b4f8-43df8e9b14d4
ORCID for Martin Christopher Grossel: ORCID iD orcid.org/0000-0001-7469-6854
ORCID for Andrew John Lotery: ORCID iD orcid.org/0000-0001-5541-4305

Catalogue record

Date deposited: 27 Apr 2010 13:25
Last modified: 14 Mar 2024 02:48

Export record

Altmetrics

Contributors

Author: Heather Anne Jane Thomson
Author: Andrew John Treharne
Author: Paul Walker

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×