Maternal diet-induced obesity alters mitochondrial activity and redox status in mouse oocytes and zygotes.
Maternal diet-induced obesity alters mitochondrial activity and redox status in mouse oocytes and zygotes.
The negative impact of obesity on reproductive success is well documented but the stages at which development of the
conceptus is compromised and the mechanisms responsible for the developmental failure still remain unclear. Recent
findings suggest that mitochondria may be a contributing factor. However to date no studies have directly addressed the
consequences of maternal obesity on mitochondria in early embryogenesis. Using an established murine model of
maternal diet induced obesity and a live cell dynamic fluorescence imaging techniques coupled with molecular biology we
have investigated the underlying mechanisms of obesity-induced reduced fertility. Our study is the first to show that
maternal obesity prior to conception is associated with altered mitochondria in mouse oocytes and zygotes. Specifically,
maternal diet-induced obesity in mice led to an increase in mitochondrial potential, mitochondrial DNA content and
biogenesis. Generation of reactive oxygen species (ROS) was raised while glutathione was depleted and the redox state
became more oxidised, suggestive of oxidative stress. These altered mitochondrial properties were associated with
significant developmental impairment as shown by the increased number of obese mothers who failed to support
blastocyst formation compared to lean dams. We propose that compromised oocyte and early embryo mitochondrial
metabolism, resulting from excessive nutrient exposure prior to and during conception, may underlie poor reproductive
outcomes frequently reported in obese women
e10074
Igosheva, Natalia
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Abramov, Andrey Y.
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Poston, Lucilla
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Eckert, Judith J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Fleming, Tom P.
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Duchen, Michael R.
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McConnell, Josie
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9 April 2010
Igosheva, Natalia
f92d39b5-c090-44ff-88b6-5c0740709845
Abramov, Andrey Y.
8fbb8a50-b8fd-4ae8-b46a-213732b3d6d9
Poston, Lucilla
916aced2-462e-445f-9efa-83ed4b7b3a9f
Eckert, Judith J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Fleming, Tom P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Duchen, Michael R.
e14608d4-e0eb-4648-8b72-82302c4b8744
McConnell, Josie
7a10a6e6-d9bb-472e-bf0b-145894c3e72d
Igosheva, Natalia, Abramov, Andrey Y., Poston, Lucilla, Eckert, Judith J., Fleming, Tom P., Duchen, Michael R. and McConnell, Josie
(2010)
Maternal diet-induced obesity alters mitochondrial activity and redox status in mouse oocytes and zygotes.
PLoS ONE, 5 (4), .
(doi:10.1371/journal.pone.0010074).
Abstract
The negative impact of obesity on reproductive success is well documented but the stages at which development of the
conceptus is compromised and the mechanisms responsible for the developmental failure still remain unclear. Recent
findings suggest that mitochondria may be a contributing factor. However to date no studies have directly addressed the
consequences of maternal obesity on mitochondria in early embryogenesis. Using an established murine model of
maternal diet induced obesity and a live cell dynamic fluorescence imaging techniques coupled with molecular biology we
have investigated the underlying mechanisms of obesity-induced reduced fertility. Our study is the first to show that
maternal obesity prior to conception is associated with altered mitochondria in mouse oocytes and zygotes. Specifically,
maternal diet-induced obesity in mice led to an increase in mitochondrial potential, mitochondrial DNA content and
biogenesis. Generation of reactive oxygen species (ROS) was raised while glutathione was depleted and the redox state
became more oxidised, suggestive of oxidative stress. These altered mitochondrial properties were associated with
significant developmental impairment as shown by the increased number of obese mothers who failed to support
blastocyst formation compared to lean dams. We propose that compromised oocyte and early embryo mitochondrial
metabolism, resulting from excessive nutrient exposure prior to and during conception, may underlie poor reproductive
outcomes frequently reported in obese women
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Published date: 9 April 2010
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Local EPrints ID: 148575
URI: http://eprints.soton.ac.uk/id/eprint/148575
ISSN: 1932-6203
PURE UUID: e4dbb105-ff36-442b-9bc6-673e2fd09933
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Date deposited: 28 Apr 2010 12:01
Last modified: 14 Mar 2024 01:03
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Contributors
Author:
Natalia Igosheva
Author:
Andrey Y. Abramov
Author:
Lucilla Poston
Author:
Michael R. Duchen
Author:
Josie McConnell
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