The impact of ovarian stimulation with recombinant FSH in combination with GnRH antagonist on the endometrial transcriptome in the window of implantation
The impact of ovarian stimulation with recombinant FSH in combination with GnRH antagonist on the endometrial transcriptome in the window of implantation
The aim of this prospective paired cohort study is to elucidate the impact of ovarian stimulation with recombinant follicle-stimulating hormone in combination with gonadotropin-releasing hormone antagonist on the endometrial transcriptome. Oocyte donors underwent endometrial biopsy during the implantation window of the nonstimulated cycle and following ovarian stimulation with recombinant follicle-stimulating hormone and gonadotropin-releasing hormone antagonist but no luteal progesterone supplementation (n = 4). Microarray analysis showed 142 genes to be significantly upregulated and 98 significantly downregulated. Significantly upregulated genes included those sequencing for the chemokine ligand CXCL 13, the Dickkopf homolog, steroidogenic acute regulatory protein, and homeobox C6. Also upregulated were genes inhibited by progesterone, such as insulin-like growth factor binding protein 5. In conclusion, ovarian stimulation with follicle-stimulating hormone and gonadotropin-releasing hormone antagonist dysregulates the expression of many genes involved in cell adhesion, T-cell receptor signaling, and regulation of signal transduction. These data suggest that dysregulation of the endometrial transcriptome in the stimulated cycle is not fully attributable to supraphysiological sex steroid levels at the folliculo-luteal transition.
endometrium, gene expression, ovarian stimulation
357-365
Macklon, Nick S.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
vaan der Gaast, Mark H.
5a54abf5-a2a5-4fbd-a8df-c70c604791cf
Hamilton, Amy
3a09c3c7-a003-4ae6-8cc9-bb4a6788c35a
Fauser, Bart C.J.M.
0edf5e0f-12db-4ca9-bf9c-00e85b7f4a3e
Giudice, Linda C.
f35bdfa2-1b26-40f4-8eff-29ec2a4c52b2
April 2008
Macklon, Nick S.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
vaan der Gaast, Mark H.
5a54abf5-a2a5-4fbd-a8df-c70c604791cf
Hamilton, Amy
3a09c3c7-a003-4ae6-8cc9-bb4a6788c35a
Fauser, Bart C.J.M.
0edf5e0f-12db-4ca9-bf9c-00e85b7f4a3e
Giudice, Linda C.
f35bdfa2-1b26-40f4-8eff-29ec2a4c52b2
Macklon, Nick S., vaan der Gaast, Mark H., Hamilton, Amy, Fauser, Bart C.J.M. and Giudice, Linda C.
(2008)
The impact of ovarian stimulation with recombinant FSH in combination with GnRH antagonist on the endometrial transcriptome in the window of implantation.
Reproductive Sciences, 15 (4), .
(doi:10.1177/1933719107311781).
Abstract
The aim of this prospective paired cohort study is to elucidate the impact of ovarian stimulation with recombinant follicle-stimulating hormone in combination with gonadotropin-releasing hormone antagonist on the endometrial transcriptome. Oocyte donors underwent endometrial biopsy during the implantation window of the nonstimulated cycle and following ovarian stimulation with recombinant follicle-stimulating hormone and gonadotropin-releasing hormone antagonist but no luteal progesterone supplementation (n = 4). Microarray analysis showed 142 genes to be significantly upregulated and 98 significantly downregulated. Significantly upregulated genes included those sequencing for the chemokine ligand CXCL 13, the Dickkopf homolog, steroidogenic acute regulatory protein, and homeobox C6. Also upregulated were genes inhibited by progesterone, such as insulin-like growth factor binding protein 5. In conclusion, ovarian stimulation with follicle-stimulating hormone and gonadotropin-releasing hormone antagonist dysregulates the expression of many genes involved in cell adhesion, T-cell receptor signaling, and regulation of signal transduction. These data suggest that dysregulation of the endometrial transcriptome in the stimulated cycle is not fully attributable to supraphysiological sex steroid levels at the folliculo-luteal transition.
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Published date: April 2008
Keywords:
endometrium, gene expression, ovarian stimulation
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Local EPrints ID: 150609
URI: http://eprints.soton.ac.uk/id/eprint/150609
ISSN: 1933-7191
PURE UUID: 882a7bbf-a98a-4db4-8cc4-467c5d3fecb4
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Date deposited: 05 May 2010 15:18
Last modified: 14 Mar 2024 01:17
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Contributors
Author:
Nick S. Macklon
Author:
Mark H. vaan der Gaast
Author:
Amy Hamilton
Author:
Bart C.J.M. Fauser
Author:
Linda C. Giudice
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