The feasibililty of a less invasive method to assess endometrial maturation--comparison of simultaneously obtained uterine secretion and tissue biopsy
The feasibililty of a less invasive method to assess endometrial maturation--comparison of simultaneously obtained uterine secretion and tissue biopsy
Objective: to compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies.
Design: prospective study.
Setting: university hospital.
Population: healthy female volunteers attending a gynaecological outpatient clinic.
Methods: endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound.
Main outcome measures: progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined. RESULTS: All data were correlated to estradiol (E2) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r=0.376, P=0.048) in endometrial fluid samples as well with serum levels of E2 (r=0.568, P=0.001) and P (r=0.408, P=0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples.
Conclusions: the measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation.
304-312
van der Gaast, M.H.
1b7e49d1-6006-4d06-a10c-499fc3e94121
Macklon, N.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Beier-Hellwig, K.
10a8c85f-165a-4e6a-a67f-310a71cb208e
Krusche, C.A.
8db9f9ee-f849-4d73-a215-1030d19c0d4f
Fauser, B.C.
a74bd2e3-1d5e-43c1-a6e8-c67c4973f484
Beier, H.M.
b5101b09-8ef4-4f30-b07a-148396194c7a
Classen-Linke, I.
055f0c95-5b4b-4e64-a7e2-f622ae7b2b1a
2009
van der Gaast, M.H.
1b7e49d1-6006-4d06-a10c-499fc3e94121
Macklon, N.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Beier-Hellwig, K.
10a8c85f-165a-4e6a-a67f-310a71cb208e
Krusche, C.A.
8db9f9ee-f849-4d73-a215-1030d19c0d4f
Fauser, B.C.
a74bd2e3-1d5e-43c1-a6e8-c67c4973f484
Beier, H.M.
b5101b09-8ef4-4f30-b07a-148396194c7a
Classen-Linke, I.
055f0c95-5b4b-4e64-a7e2-f622ae7b2b1a
van der Gaast, M.H., Macklon, N., Beier-Hellwig, K., Krusche, C.A., Fauser, B.C., Beier, H.M. and Classen-Linke, I.
(2009)
The feasibililty of a less invasive method to assess endometrial maturation--comparison of simultaneously obtained uterine secretion and tissue biopsy.
BJOG: An International Journal of Obstetrics & Gynaecology, 116 (2), .
(doi:10.1111/j.1471-0528.2008.02039.x).
Abstract
Objective: to compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies.
Design: prospective study.
Setting: university hospital.
Population: healthy female volunteers attending a gynaecological outpatient clinic.
Methods: endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound.
Main outcome measures: progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined. RESULTS: All data were correlated to estradiol (E2) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r=0.376, P=0.048) in endometrial fluid samples as well with serum levels of E2 (r=0.568, P=0.001) and P (r=0.408, P=0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples.
Conclusions: the measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation.
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Published date: 2009
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Local EPrints ID: 150621
URI: http://eprints.soton.ac.uk/id/eprint/150621
ISSN: 1470-0328
PURE UUID: c36b908d-5024-4c90-9796-d7a22765ac1d
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Date deposited: 05 May 2010 15:45
Last modified: 14 Mar 2024 01:17
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Author:
M.H. van der Gaast
Author:
N. Macklon
Author:
K. Beier-Hellwig
Author:
C.A. Krusche
Author:
B.C. Fauser
Author:
H.M. Beier
Author:
I. Classen-Linke
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