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Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity

Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity
Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity
Investigation of human embryo implantation requires a non-disruptive means of studying the endometrium during the window of implantation. This study describes a novel approach of cytokine profiling in endometrial secretions. Endometrial secretions aspirated prior to embryo transfer from 210 women undergoing IVF or intracytoplasmic sperm injection were analysed by a multiplex immunoassay. Ten mediators [interleukin (IL)-1beta, IL-6, IL-12, IL-18, tumour necrosis factor-alpha, macrophage migration inhibitory factor, eotaxin, monocyte chemotactic protein-1, interferon-gamma inducible protein-10, vascular endothelial growth factor] were detectable in 90-100% of the samples. Heparin-binding epidermal growth factor, IL-5, IL-17, IL-10, Dickkopf homologue-1 and IL-15 were detected in 23-76%, whereas interferon-gamma was not detectable in any of the samples. To assess possible contamination of samples, cervical mucus was also aspirated for comparative analysis in 22 women. The endometrial cytokine profile differed significantly from cervical mucus. Pregnancy rates of the study participants who underwent endometrial secretion aspiration were compared with 210 controls matched for important prognostic variables; no significant differences were found. In conclusion, cytokine profiling in endometrial secretion offers an objective, non-disruptive means of analysing the in-vivo milieu encountered by the embryo and offers a new and potentially valuable approach to studying the endometrial factor in human embryo implantation.

cytokines, endometrial receptivity, endometrial secretion, ivf
85-94
Boomsma, C.M.
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Kavelaars, A.
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Eijkemans, M.J.C.
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Amarouchi, K.
b96ba628-88a0-4c42-b214-8938bc0e74d3
Teklenburg, G.
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Gutknecht, D.
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Fauser, B.C.J.M.
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Heijnen, C.J.
d06ef259-793d-474c-8728-ab3ec6cc8946
Macklon, N.
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Boomsma, C.M.
52d1e262-30d1-47dc-af36-ae0be5c2e6e0
Kavelaars, A.
e26bbe8f-5e49-4a0f-897e-4e1441657010
Eijkemans, M.J.C.
49ac87bc-76a5-493a-8d2b-37abfb606e62
Amarouchi, K.
b96ba628-88a0-4c42-b214-8938bc0e74d3
Teklenburg, G.
4575b926-790e-4d72-a444-9bd8df4fdca9
Gutknecht, D.
5df843d0-f185-4c69-aa27-5a51588ed945
Fauser, B.C.J.M.
c265d1e7-d207-4400-a669-7502343e3b7e
Heijnen, C.J.
d06ef259-793d-474c-8728-ab3ec6cc8946
Macklon, N.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e

Boomsma, C.M., Kavelaars, A., Eijkemans, M.J.C., Amarouchi, K., Teklenburg, G., Gutknecht, D., Fauser, B.C.J.M., Heijnen, C.J. and Macklon, N. (2009) Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity. Reproductive BioMedicine Online, 18 (1), 85-94. (doi:10.1016/S1472-6483(10)60429-4).

Record type: Article

Abstract

Investigation of human embryo implantation requires a non-disruptive means of studying the endometrium during the window of implantation. This study describes a novel approach of cytokine profiling in endometrial secretions. Endometrial secretions aspirated prior to embryo transfer from 210 women undergoing IVF or intracytoplasmic sperm injection were analysed by a multiplex immunoassay. Ten mediators [interleukin (IL)-1beta, IL-6, IL-12, IL-18, tumour necrosis factor-alpha, macrophage migration inhibitory factor, eotaxin, monocyte chemotactic protein-1, interferon-gamma inducible protein-10, vascular endothelial growth factor] were detectable in 90-100% of the samples. Heparin-binding epidermal growth factor, IL-5, IL-17, IL-10, Dickkopf homologue-1 and IL-15 were detected in 23-76%, whereas interferon-gamma was not detectable in any of the samples. To assess possible contamination of samples, cervical mucus was also aspirated for comparative analysis in 22 women. The endometrial cytokine profile differed significantly from cervical mucus. Pregnancy rates of the study participants who underwent endometrial secretion aspiration were compared with 210 controls matched for important prognostic variables; no significant differences were found. In conclusion, cytokine profiling in endometrial secretion offers an objective, non-disruptive means of analysing the in-vivo milieu encountered by the embryo and offers a new and potentially valuable approach to studying the endometrial factor in human embryo implantation.

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More information

Published date: January 2009
Keywords: cytokines, endometrial receptivity, endometrial secretion, ivf

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Local EPrints ID: 150725
URI: https://eprints.soton.ac.uk/id/eprint/150725
PURE UUID: d10a6351-2165-4d2d-9497-c0518a60a038

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Date deposited: 06 May 2010 11:48
Last modified: 18 Jul 2017 12:56

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Contributors

Author: C.M. Boomsma
Author: A. Kavelaars
Author: M.J.C. Eijkemans
Author: K. Amarouchi
Author: G. Teklenburg
Author: D. Gutknecht
Author: B.C.J.M. Fauser
Author: C.J. Heijnen
Author: N. Macklon

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