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Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach

Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach
Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach
Background: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.

Results: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.

Conclusion: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.
1742-4682
Harney, D.F.
6f1f0a73-f25a-4dd4-bb5a-f06390c65639
Butler, R.
e164104d-4531-46e6-8861-377e8502197e
Edwards, Richard J.
9d25e74f-dc0d-455a-832c-5f363d864c43
Harney, D.F.
6f1f0a73-f25a-4dd4-bb5a-f06390c65639
Butler, R.
e164104d-4531-46e6-8861-377e8502197e
Edwards, Richard J.
9d25e74f-dc0d-455a-832c-5f363d864c43

Harney, D.F., Butler, R. and Edwards, Richard J. (2005) Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach. Theoretical Biology & Medical Modelling, 2 (12). (doi:10.1186/1742-4682-2-12).

Record type: Article

Abstract

Background: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.

Results: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.

Conclusion: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.

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Published date: 25 March 2005

Identifiers

Local EPrints ID: 151151
URI: https://eprints.soton.ac.uk/id/eprint/151151
ISSN: 1742-4682
PURE UUID: 423e13f1-f610-4ce5-bc85-ca59c23bd7d6

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Date deposited: 28 Jun 2010 12:20
Last modified: 18 Jul 2017 12:55

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Contributors

Author: D.F. Harney
Author: R. Butler
Author: Richard J. Edwards

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