Identification of invasive serotype 1 pneumococcal isolates that express nonhemolytic pneumolysin
Identification of invasive serotype 1 pneumococcal isolates that express nonhemolytic pneumolysin
Recently, there has been an increase in invasive pneumococcal disease (IPD) caused by serotype 1 Streptococcus pneumoniae throughout Europe. Serotype 1 IPD is associated with bacteremia and pneumonia in Europe and North America, especially in neonates, and is ranked among the top five most prevalent pneumococcal serotypes in at least 10 countries. The currently licensed pediatric pneumococcal vaccine does not afford protection to this serotype. Upon screening of 252 clinical isolates of S. pneumoniae, we discovered mutations in the pneumolysin gene of two out of the four serotype 1 strains present in the study group. Analysis of an additional 28 serotype 1 isolates from patients with IPD from various Scottish Health Boards, revealed that >50% had mutations in their pneumolysin genes. This resulted in the expression of nonhemolytic forms of pneumolysin. All of the strains producing nonhemolytic pneumolysin were sequence type 306 (ST306), whereas those producing "wild-type" pneumolysin were ST227. The mutations were in a region of pneumolysin involved in pore formation. These mutations can be made in vitro to give the nonhemolytic phenotype. Pneumolysin is generally conserved throughout all serotypes of S. pneumoniae and is essential for full invasive disease; however, it appears that serotype 1 ST306 does not require hemolytically active pneumolysin to cause IPD.
151-159
Kirkham, Lea-Ann S.
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Jefferies, Johanna M.C.
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Kerr, Alison R.
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Jing, Yu
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Clarke, Stuart C.
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Smith, Andrew
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Mitchell, Tim J.
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January 2006
Kirkham, Lea-Ann S.
ad6fd8f8-955e-4089-99af-1f7d36f1de30
Jefferies, Johanna M.C.
942356b7-2070-49ad-9376-cde03c5fe13d
Kerr, Alison R.
aa73bf04-62f2-4a87-8ff4-09c8f057e697
Jing, Yu
22a5299e-e26e-4392-903d-8dbaa288a049
Clarke, Stuart C.
6a26cdfc-8fee-456f-a5ae-9c16db5545b0
Smith, Andrew
28d2a985-1801-4e42-9a43-8061526d8e48
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Kirkham, Lea-Ann S., Jefferies, Johanna M.C., Kerr, Alison R., Jing, Yu, Clarke, Stuart C., Smith, Andrew and Mitchell, Tim J.
(2006)
Identification of invasive serotype 1 pneumococcal isolates that express nonhemolytic pneumolysin.
Journal of Clinical Microbiology, 44 (1), .
(doi:10.1128/JCM.44.1.151-159.2006).
Abstract
Recently, there has been an increase in invasive pneumococcal disease (IPD) caused by serotype 1 Streptococcus pneumoniae throughout Europe. Serotype 1 IPD is associated with bacteremia and pneumonia in Europe and North America, especially in neonates, and is ranked among the top five most prevalent pneumococcal serotypes in at least 10 countries. The currently licensed pediatric pneumococcal vaccine does not afford protection to this serotype. Upon screening of 252 clinical isolates of S. pneumoniae, we discovered mutations in the pneumolysin gene of two out of the four serotype 1 strains present in the study group. Analysis of an additional 28 serotype 1 isolates from patients with IPD from various Scottish Health Boards, revealed that >50% had mutations in their pneumolysin genes. This resulted in the expression of nonhemolytic forms of pneumolysin. All of the strains producing nonhemolytic pneumolysin were sequence type 306 (ST306), whereas those producing "wild-type" pneumolysin were ST227. The mutations were in a region of pneumolysin involved in pore formation. These mutations can be made in vitro to give the nonhemolytic phenotype. Pneumolysin is generally conserved throughout all serotypes of S. pneumoniae and is essential for full invasive disease; however, it appears that serotype 1 ST306 does not require hemolytically active pneumolysin to cause IPD.
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Published date: January 2006
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Local EPrints ID: 151789
URI: http://eprints.soton.ac.uk/id/eprint/151789
ISSN: 0095-1137
PURE UUID: 7bf88cbc-0c41-4948-96b9-6372de423925
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Date deposited: 18 Jun 2010 09:08
Last modified: 14 Mar 2024 01:21
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Contributors
Author:
Lea-Ann S. Kirkham
Author:
Johanna M.C. Jefferies
Author:
Alison R. Kerr
Author:
Yu Jing
Author:
Stuart C. Clarke
Author:
Andrew Smith
Author:
Tim J. Mitchell
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