A pilot study of Connexin 43 (Cx43) in human bladder tissue patients with idiopathic detrusor overactivity
A pilot study of Connexin 43 (Cx43) in human bladder tissue patients with idiopathic detrusor overactivity
Objective: aim of the study was to compare Connexin 43 (Cx43) in human bladder tissue of urodynamically proven idiopathic detrusor overactivity to those of urodynamically stable bladders.
Study design: we compared bladder biopsies of patients with detrusor overactivity and those with stable bladder analysing Cx43 message by RNA extraction and PCR amplification. All patients had multichannel urodynamics prior to the biopsies.
Results: we investigated the bladder biopsies of 15 female patients with and 15 patients without detrusor overactivity. Cx43 could be detected in nine patients of the detrusor overactivity group and in eight patients of the control group which was not statistically significant. 42 cycles of PCR were necessary to demonstrate Cx43 presence in the positive specimen. The presence of Cx43 was not consistent in the samples from the bladder dome and the side walls meaning there were Cx43 positive results in the dome and negative ones in the side walls of the same patient and vice versa.
Conclusion: in conclusion, Cx43 is present in human bladder tissue both of overactive bladders and those of controls. However, it is expressed in very small amounts and is not always detectable. The role of Cx43 for the origin of detrusor overactivity remains unclear.
Cx43, overactive bladder syndrome, incontinence, bladder capacity
83-86
Kuhn, Annette
5bf35057-4ff3-4d5a-b08f-3c68515ae357
Stadlmayr, Werner
bc0bc973-62ad-4d76-b6d2-d37fbe0348ee
Monga, Ash
60b415bd-97b3-485b-b641-0751151036d3
Cameron, Ian
f7595539-efa6-4687-b161-e1e93ff710f2
Anthony, Fred
6b170ff0-0c4c-4930-9802-93cec20b776a
November 2008
Kuhn, Annette
5bf35057-4ff3-4d5a-b08f-3c68515ae357
Stadlmayr, Werner
bc0bc973-62ad-4d76-b6d2-d37fbe0348ee
Monga, Ash
60b415bd-97b3-485b-b641-0751151036d3
Cameron, Ian
f7595539-efa6-4687-b161-e1e93ff710f2
Anthony, Fred
6b170ff0-0c4c-4930-9802-93cec20b776a
Kuhn, Annette, Stadlmayr, Werner, Monga, Ash, Cameron, Ian and Anthony, Fred
(2008)
A pilot study of Connexin 43 (Cx43) in human bladder tissue patients with idiopathic detrusor overactivity.
European Journal of Obstetrics, Gynecology and Reproductive Biology, 141 (1), .
(doi:10.1016/j.ejogrb.2008.06.002).
Abstract
Objective: aim of the study was to compare Connexin 43 (Cx43) in human bladder tissue of urodynamically proven idiopathic detrusor overactivity to those of urodynamically stable bladders.
Study design: we compared bladder biopsies of patients with detrusor overactivity and those with stable bladder analysing Cx43 message by RNA extraction and PCR amplification. All patients had multichannel urodynamics prior to the biopsies.
Results: we investigated the bladder biopsies of 15 female patients with and 15 patients without detrusor overactivity. Cx43 could be detected in nine patients of the detrusor overactivity group and in eight patients of the control group which was not statistically significant. 42 cycles of PCR were necessary to demonstrate Cx43 presence in the positive specimen. The presence of Cx43 was not consistent in the samples from the bladder dome and the side walls meaning there were Cx43 positive results in the dome and negative ones in the side walls of the same patient and vice versa.
Conclusion: in conclusion, Cx43 is present in human bladder tissue both of overactive bladders and those of controls. However, it is expressed in very small amounts and is not always detectable. The role of Cx43 for the origin of detrusor overactivity remains unclear.
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More information
Published date: November 2008
Keywords:
Cx43, overactive bladder syndrome, incontinence, bladder capacity
Identifiers
Local EPrints ID: 152221
URI: http://eprints.soton.ac.uk/id/eprint/152221
ISSN: 0301-2115
PURE UUID: 7f43c060-75c9-42be-bfc2-b2f58434c3c3
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Date deposited: 13 May 2010 15:12
Last modified: 14 Mar 2024 02:42
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Author:
Annette Kuhn
Author:
Werner Stadlmayr
Author:
Ash Monga
Author:
Fred Anthony
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