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Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men

Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men
Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men
This study investigated the incorporation of cis-9,trans-11 conjugated linoleic acid (c9,t11 CLA) and trans-10,cis-12-CLA (t10,c12 CLA) into plasma and peripheral blood mononuclear cell (PBMC) lipids when consumed as supplements highly enriched in these isomers. Healthy men (n = 49, age 31 ± 8 years) consumed one, two, and four capsules containing ~600 mg of either c9,t11 CLA or t10,c12 CLA per capsule for sequential 8 week periods followed by a 6 week washout before consuming the alternative isomer. Both isomers were incorporated in a dose-dependent manner into plasma phosphatidylcholine (PC) (c9,t11 CLA r = 0.779, t10,c12 CLA r = 0.738; P < 0.0001) and cholesteryl ester (CE) (c9,t11 CLA r = 0.706, t10,c12 CLA r = 0.788; P < 0.0001). Only t10,c12 CLA was enriched in plasma nonesterified fatty acids. Both c9,t11 CLA and t10,c12 CLA were incorporated linearly into PBMC total lipids (r = 0.285 and r = 0.273, respectively; P < 0.0005). The highest concentrations of c9,t11 CLA and t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than those in plasma PC and CE.
These data suggest that the level of intake is a major determinant of plasma and PBMC CLA content, although PBMCs appear to incorporate both CLA isomers less readily.
phosphatidylcholine, cholesteryl ester, nonesterified fatty acids, leukocyte, human
0022-2275
736-741
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Lupoli, Berit
b6a9e4fc-c7a1-47ef-91a9-0ad31dd681aa
Russell, Jennifer J.
54a00b9a-1957-4583-ae3d-0ad1a8b4e016
Tricon, Sabine
4e65d971-4693-471f-bc7f-543c631b0363
Kew, Samantha
0388d70a-7691-4660-9896-95a45099a5db
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Shingfield, Kevin J.
3c256b2d-ea7a-4ff0-b571-c9550d32922c
Beever, David E.
2c1f23a4-f98f-4fdf-89a1-fb3f2831f219
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Williams, Christine M.
4cf5b7be-8973-4ed3-9150-5caf74999670
Yaqoob, Parveen
3a418e24-bbf0-4b31-9df8-ca8514885c82
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Lupoli, Berit
b6a9e4fc-c7a1-47ef-91a9-0ad31dd681aa
Russell, Jennifer J.
54a00b9a-1957-4583-ae3d-0ad1a8b4e016
Tricon, Sabine
4e65d971-4693-471f-bc7f-543c631b0363
Kew, Samantha
0388d70a-7691-4660-9896-95a45099a5db
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Shingfield, Kevin J.
3c256b2d-ea7a-4ff0-b571-c9550d32922c
Beever, David E.
2c1f23a4-f98f-4fdf-89a1-fb3f2831f219
Grimble, Robert F.
3100e4d2-8f29-4ca6-a95d-38a6a764865f
Williams, Christine M.
4cf5b7be-8973-4ed3-9150-5caf74999670
Yaqoob, Parveen
3a418e24-bbf0-4b31-9df8-ca8514885c82
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Burdge, Graham C., Lupoli, Berit, Russell, Jennifer J., Tricon, Sabine, Kew, Samantha, Banerjee, Tapati, Shingfield, Kevin J., Beever, David E., Grimble, Robert F., Williams, Christine M., Yaqoob, Parveen and Calder, Philip C. (2004) Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men. Journal of Lipid Research, 45 (4), 736-741. (doi:10.1194/jlr.M300447-JLR200).

Record type: Article

Abstract

This study investigated the incorporation of cis-9,trans-11 conjugated linoleic acid (c9,t11 CLA) and trans-10,cis-12-CLA (t10,c12 CLA) into plasma and peripheral blood mononuclear cell (PBMC) lipids when consumed as supplements highly enriched in these isomers. Healthy men (n = 49, age 31 ± 8 years) consumed one, two, and four capsules containing ~600 mg of either c9,t11 CLA or t10,c12 CLA per capsule for sequential 8 week periods followed by a 6 week washout before consuming the alternative isomer. Both isomers were incorporated in a dose-dependent manner into plasma phosphatidylcholine (PC) (c9,t11 CLA r = 0.779, t10,c12 CLA r = 0.738; P < 0.0001) and cholesteryl ester (CE) (c9,t11 CLA r = 0.706, t10,c12 CLA r = 0.788; P < 0.0001). Only t10,c12 CLA was enriched in plasma nonesterified fatty acids. Both c9,t11 CLA and t10,c12 CLA were incorporated linearly into PBMC total lipids (r = 0.285 and r = 0.273, respectively; P < 0.0005). The highest concentrations of c9,t11 CLA and t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than those in plasma PC and CE.
These data suggest that the level of intake is a major determinant of plasma and PBMC CLA content, although PBMCs appear to incorporate both CLA isomers less readily.

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More information

Published date: 1 April 2004
Keywords: phosphatidylcholine, cholesteryl ester, nonesterified fatty acids, leukocyte, human
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 152459
URI: https://eprints.soton.ac.uk/id/eprint/152459
ISSN: 0022-2275
PURE UUID: 7f4cc04c-31c7-41d3-b8c3-e321bf253485
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

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Date deposited: 27 May 2011 10:19
Last modified: 06 Oct 2018 00:39

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Contributors

Author: Berit Lupoli
Author: Jennifer J. Russell
Author: Sabine Tricon
Author: Samantha Kew
Author: Tapati Banerjee
Author: Kevin J. Shingfield
Author: David E. Beever
Author: Robert F. Grimble
Author: Christine M. Williams
Author: Parveen Yaqoob

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