Automation of MLST using third-generation liquid-handling technology
Automation of MLST using third-generation liquid-handling technology
The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on Neisseria meningitidis, Streptococcus pneumonaie, Haemophilus influenzae, and Staphylococcus aureus. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting. Here we describe the automation of MLST on a third-generation liquid-handling robot.
automation, MLST, meningococci, pneumococci, typing, molecular characterization
219-225
Sullivan, C.B.
ea705922-584f-40c1-b982-348dc0e14563
Jefferies, J.M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Diggle, M.A.
eedad686-5161-477b-b497-94a90ac1f237
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
March 2006
Sullivan, C.B.
ea705922-584f-40c1-b982-348dc0e14563
Jefferies, J.M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Diggle, M.A.
eedad686-5161-477b-b497-94a90ac1f237
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Sullivan, C.B., Jefferies, J.M.C., Diggle, M.A. and Clarke, S.C.
(2006)
Automation of MLST using third-generation liquid-handling technology.
Molecular Biotechnology, 32 (3), .
(doi:10.1385/MB:32:3:219).
Abstract
The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on Neisseria meningitidis, Streptococcus pneumonaie, Haemophilus influenzae, and Staphylococcus aureus. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting. Here we describe the automation of MLST on a third-generation liquid-handling robot.
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Published date: March 2006
Keywords:
automation, MLST, meningococci, pneumococci, typing, molecular characterization
Identifiers
Local EPrints ID: 152813
URI: http://eprints.soton.ac.uk/id/eprint/152813
ISSN: 1073-6085
PURE UUID: f7fa2272-3633-490f-adc0-a8c558da5248
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Date deposited: 09 Jun 2010 12:56
Last modified: 14 Mar 2024 02:51
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Contributors
Author:
C.B. Sullivan
Author:
J.M.C. Jefferies
Author:
M.A. Diggle
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