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Genomic diversity between strains of the same serotype and Multilocus sequence type among pneumococcal clinical isolates

Genomic diversity between strains of the same serotype and Multilocus sequence type among pneumococcal clinical isolates
Genomic diversity between strains of the same serotype and Multilocus sequence type among pneumococcal clinical isolates
The important human pathogen Streptococcus pneumoniae is known to be a genetically diverse species. We have
used comparative genome hybridization (CGH) microarray analysis to investigate this diversity in a collection of
clinical isolates including several capsule serotype 14 pneumococci, a dominant serotype among disease isolates. We
have identified three new regions of diversity among pneumococcal isolates and, importantly, clearly demonstrate
genetic differences between strains of the same multilocus sequence type (ST) and capsule serotype. CGH may
therefore, under certain circumstances, prove to be a valuable tool to supplement current typing methods. Finally,
we show that these clonal strains with the same serotype and ST behave differently in an animal model. Strains of
the same ST and serotype therefore have important genetic and phenotypic differences.
0019-9567
3513-3518
Silva, Nuno A.
acb6a543-b9a8-419b-8570-43f862ad3667
McClusky, Jackie
c9bdab93-4341-4b43-804c-1a0d33c26e50
Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Hinds, Johanna M.C.
fea2b203-2893-450a-a05a-a86ddec39f74
Smith, Andrew
28d2a985-1801-4e42-9a43-8061526d8e48
Clarke, Stuart C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Paterson, Gavin K.
23fb570a-f575-4163-95f6-8a9a1c58563b
Silva, Nuno A.
acb6a543-b9a8-419b-8570-43f862ad3667
McClusky, Jackie
c9bdab93-4341-4b43-804c-1a0d33c26e50
Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Hinds, Johanna M.C.
fea2b203-2893-450a-a05a-a86ddec39f74
Smith, Andrew
28d2a985-1801-4e42-9a43-8061526d8e48
Clarke, Stuart C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Paterson, Gavin K.
23fb570a-f575-4163-95f6-8a9a1c58563b

Silva, Nuno A., McClusky, Jackie, Jefferies, Johanna M.C., Hinds, Johanna M.C., Smith, Andrew, Clarke, Stuart C., Mitchell, Tim J. and Paterson, Gavin K. (2006) Genomic diversity between strains of the same serotype and Multilocus sequence type among pneumococcal clinical isolates. Infection and Immunity, 74 (6), 3513-3518. (doi:10.1128/IAI.00079-06).

Record type: Article

Abstract

The important human pathogen Streptococcus pneumoniae is known to be a genetically diverse species. We have
used comparative genome hybridization (CGH) microarray analysis to investigate this diversity in a collection of
clinical isolates including several capsule serotype 14 pneumococci, a dominant serotype among disease isolates. We
have identified three new regions of diversity among pneumococcal isolates and, importantly, clearly demonstrate
genetic differences between strains of the same multilocus sequence type (ST) and capsule serotype. CGH may
therefore, under certain circumstances, prove to be a valuable tool to supplement current typing methods. Finally,
we show that these clonal strains with the same serotype and ST behave differently in an animal model. Strains of
the same ST and serotype therefore have important genetic and phenotypic differences.

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Published date: June 2006

Identifiers

Local EPrints ID: 152815
URI: http://eprints.soton.ac.uk/id/eprint/152815
ISSN: 0019-9567
PURE UUID: 4add7418-2c3d-41a9-af2e-b5e710651586
ORCID for Stuart C. Clarke: ORCID iD orcid.org/0000-0002-7009-1548

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Date deposited: 22 Jun 2010 14:15
Last modified: 14 Mar 2024 02:51

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Contributors

Author: Nuno A. Silva
Author: Jackie McClusky
Author: Johanna M.C. Jefferies
Author: Johanna M.C. Hinds
Author: Andrew Smith
Author: Tim J. Mitchell
Author: Gavin K. Paterson

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