Presence of nonhemolytic pneumolysin in serotypes of streptococcus pneumoniae associated with disease outbreaks
Presence of nonhemolytic pneumolysin in serotypes of streptococcus pneumoniae associated with disease outbreaks
Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty?two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak?associated serotypes of S. pneumoniae.
936-944
Jefferies, Johanna M.C.
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Johnston, Calum H.G.
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Kirkham, Lea-Ann S.
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Cowan, Graeham J.M.
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Ross, Kirsty S.
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Smith, Andrew
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Clarke, Stuart C.
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Brueggeman, Angela B.
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George, Robert C.
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Pichon, Bruno
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Pluschke, Gerd
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Pfluger, Valentin
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Mitchell, Tim J.
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15 September 2007
Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Johnston, Calum H.G.
53c91b09-9cbe-4853-8854-b48c425105fb
Kirkham, Lea-Ann S.
ad6fd8f8-955e-4089-99af-1f7d36f1de30
Cowan, Graeham J.M.
327fa306-217c-4daf-9dcd-0f26f580a84d
Ross, Kirsty S.
d37752c2-b85f-4669-93bd-87819462dde9
Smith, Andrew
28d2a985-1801-4e42-9a43-8061526d8e48
Clarke, Stuart C.
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Brueggeman, Angela B.
8333a26f-730f-47c4-8d2c-5c2ffb89a480
George, Robert C.
982abdc9-34fa-43b0-9544-324f046455b5
Pichon, Bruno
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Pluschke, Gerd
16ecb115-c409-491b-a8a4-418023f3ba63
Pfluger, Valentin
3aeac4a4-96b2-4032-b2e9-cce5d97fc1f3
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Jefferies, Johanna M.C., Johnston, Calum H.G., Kirkham, Lea-Ann S., Cowan, Graeham J.M., Ross, Kirsty S., Smith, Andrew, Clarke, Stuart C., Brueggeman, Angela B., George, Robert C., Pichon, Bruno, Pluschke, Gerd, Pfluger, Valentin and Mitchell, Tim J.
(2007)
Presence of nonhemolytic pneumolysin in serotypes of streptococcus pneumoniae associated with disease outbreaks.
Journal of Infectious Diseases, 196, .
(doi:10.1086/520091).
Abstract
Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty?two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak?associated serotypes of S. pneumoniae.
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520091
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Published date: 15 September 2007
Additional Information:
PubMed ID: 17703426
Organisations:
Infection Inflammation & Immunity
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Local EPrints ID: 153089
URI: http://eprints.soton.ac.uk/id/eprint/153089
ISSN: 0022-1899
PURE UUID: 10ff1980-9766-4544-86c8-a939700bb521
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Date deposited: 03 Jun 2010 09:08
Last modified: 14 Mar 2024 02:51
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Contributors
Author:
Johanna M.C. Jefferies
Author:
Calum H.G. Johnston
Author:
Lea-Ann S. Kirkham
Author:
Graeham J.M. Cowan
Author:
Kirsty S. Ross
Author:
Andrew Smith
Author:
Angela B. Brueggeman
Author:
Robert C. George
Author:
Bruno Pichon
Author:
Gerd Pluschke
Author:
Valentin Pfluger
Author:
Tim J. Mitchell
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