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Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan

Wu, Ai-Min, Hornblad, Emma, Voxeur, Aline, Gerber, Lorenz, Rihouey, Christophe, Lerouge, Patrice and Marchant, Alan (2010) Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan Plant Physiology, 153, (2), pp. 542-554. (doi:10.1104/pp.110.154971).

Record type: Article

Abstract

The hemicellulose glucuronoxylan (GX) is a major component of plant secondary cell walls. However, our understanding of GX synthesis remains limited. Here, we identify and analyze two new genes from Arabidopsis (Arabidopsis thaliana), IRREGULAR XYLEM9-LIKE (IRX9-L) and IRX14-LIKE (IRX14-L) that encode glycosyltransferase family 43 members proposed to function during xylan backbone elongation. We place IRX9-L and IRX14-L in a genetic framework with six previously described glycosyltransferase genes (IRX9, IRX10, IRX10-L, IRX14, FRAGILE FIBER8 [FRA8], and FRA8 HOMOLOG [F8H]) and investigate their function in GX synthesis. Double-mutant analysis identifies IRX9-L and IRX14-L as functional homologs of IRX9 and IRX14, respectively. Characterization of irx9 irx10 irx14 fra8 and irx9-L irx10-L irx14-L f8h quadruple mutants allows definition of a set of genes comprising IRX9, IRX10, IRX14, and FRA8 that perform the main role in GX synthesis during vegetative development. The IRX9-L, IRX10-L, IRX14-L, and F8H genes are able to partially substitute for their respective homologs and normally perform a minor function. The irx14 irx14-L double mutant virtually lacks xylan, whereas irx9 irx9-L and fra8 f8h double mutants form lowered amounts of GX displaying a greatly reduced degree of backbone polymerization. Our findings reveal two distinct sets of four genes each differentially contributing to GX biosynthesis.

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Published date: June 2010
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 154933
URI: http://eprints.soton.ac.uk/id/eprint/154933
ISSN: 0032-0889
PURE UUID: 074d9428-695b-45df-9abb-126c78497e94

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Date deposited: 26 May 2010 13:14
Last modified: 18 Jul 2017 12:46

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Contributors

Author: Ai-Min Wu
Author: Emma Hornblad
Author: Aline Voxeur
Author: Lorenz Gerber
Author: Christophe Rihouey
Author: Patrice Lerouge
Author: Alan Marchant

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