Cloning and expression analysis of drosophila melanogaster extracellular Cu Zn SOD reveals sex specific variation
Cloning and expression analysis of drosophila melanogaster extracellular Cu Zn SOD reveals sex specific variation
Abstract: The superoxide dismutase (SOD) family of enzymes regulate reactive oxygen species by scavenging superoxide. In Drosophila homologues of two of the mammalian SODs, the intracellular (Sod1) and mitochondrial (Sod2) SODs, have been studied extensively indicating a link between SOD and ageing. Recently the sequence of a third Sod gene, homologous to both mammalian (Sod3) and C. elegans (Sod4) extracellular Sod, was identified in Drosophila and is also predicted to locate extracellularly (Sod3). We have cloned and sequenced this gene, identifying two gene products formed by alternative splicing. Here we report differential sex specific Sod3 expression patterns along with validation of a Sod3 hypomorph mutant. Furthermore, we indicate a functional role of Sod3 in protecting against H2O2 induced oxidative stress.
Blackney, Michael J.
748d1912-5439-463d-a4c8-459912c2a164
Shepherd, D.
a12a1d37-2cdc-47d9-84f6-efdd8096f51d
Parker, J.D.
34055903-007a-42d0-b1af-2717f217ab41
2010
Blackney, Michael J.
748d1912-5439-463d-a4c8-459912c2a164
Shepherd, D.
a12a1d37-2cdc-47d9-84f6-efdd8096f51d
Parker, J.D.
34055903-007a-42d0-b1af-2717f217ab41
Blackney, Michael J., Shepherd, D. and Parker, J.D.
(2010)
Cloning and expression analysis of drosophila melanogaster extracellular Cu Zn SOD reveals sex specific variation.
Journal of Insect Molecular Biology.
Abstract
Abstract: The superoxide dismutase (SOD) family of enzymes regulate reactive oxygen species by scavenging superoxide. In Drosophila homologues of two of the mammalian SODs, the intracellular (Sod1) and mitochondrial (Sod2) SODs, have been studied extensively indicating a link between SOD and ageing. Recently the sequence of a third Sod gene, homologous to both mammalian (Sod3) and C. elegans (Sod4) extracellular Sod, was identified in Drosophila and is also predicted to locate extracellularly (Sod3). We have cloned and sequenced this gene, identifying two gene products formed by alternative splicing. Here we report differential sex specific Sod3 expression patterns along with validation of a Sod3 hypomorph mutant. Furthermore, we indicate a functional role of Sod3 in protecting against H2O2 induced oxidative stress.
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Published date: 2010
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Local EPrints ID: 154969
URI: http://eprints.soton.ac.uk/id/eprint/154969
ISSN: 0962-1075
PURE UUID: 12df8dd1-0e5e-4d35-8e92-fcc064d7cea2
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Date deposited: 26 May 2010 14:03
Last modified: 10 Dec 2021 18:12
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Author:
Michael J. Blackney
Author:
D. Shepherd
Author:
J.D. Parker
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