Blackney, Michael J., Shepherd, D. and Parker, J.D.
Cloning and expression analysis of drosophila melanogaster extracellular Cu Zn SOD reveals sex specific variation
Journal of Insect Molecular Biology
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Abstract: The superoxide dismutase (SOD) family of enzymes regulate reactive oxygen species by scavenging superoxide. In Drosophila homologues of two of the mammalian SODs, the intracellular (Sod1) and mitochondrial (Sod2) SODs, have been studied extensively indicating a link between SOD and ageing. Recently the sequence of a third Sod gene, homologous to both mammalian (Sod3) and C. elegans (Sod4) extracellular Sod, was identified in Drosophila and is also predicted to locate extracellularly (Sod3). We have cloned and sequenced this gene, identifying two gene products formed by alternative splicing. Here we report differential sex specific Sod3 expression patterns along with validation of a Sod3 hypomorph mutant. Furthermore, we indicate a functional role of Sod3 in protecting against H2O2 induced oxidative stress.
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