The University of Southampton
University of Southampton Institutional Repository

Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device

Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device
Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device
A micromachined capillary electrophoresis system has been fabricated on a glass device for the separation and indirect fluorescence detection of phenols. Using this device two phenols viz., 2,4-dichlorophenol and pentachlorophenol, were separated within 12 s compared to under 19 min on a conventional capillary electrophoresis system using direct ultraviolet detection. The precision of the glass device ranged from 12.7%–16.7% compared to 0.42%–4.9% for the conventional system. Both systems showed good linearity in the concentration range of 0.8– 6.38 mM for the glass device and 5–130 ?M for the conventional system. The relationship between temperature and high voltage with baseline drift was also investigated. These results provide a foundation for the development of a miniaturised chemical analysis system for the on-line analysis of phenols in water.
1432-1130
686-691
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Whalley, Peter D.
2b0de185-77ca-46c3-9757-9dbb24583f94
Manz, A.
d8d789e5-7066-4583-81f2-9d0410d3bbdc
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Whalley, Peter D.
2b0de185-77ca-46c3-9757-9dbb24583f94
Manz, A.
d8d789e5-7066-4583-81f2-9d0410d3bbdc

Arundell, Martin, Whalley, Peter D. and Manz, A. (2000) Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device. Fresenius' Journal of Analytical Chemistry, 367 (8), 686-691. (doi:10.1007/s002160000460).

Record type: Article

Abstract

A micromachined capillary electrophoresis system has been fabricated on a glass device for the separation and indirect fluorescence detection of phenols. Using this device two phenols viz., 2,4-dichlorophenol and pentachlorophenol, were separated within 12 s compared to under 19 min on a conventional capillary electrophoresis system using direct ultraviolet detection. The precision of the glass device ranged from 12.7%–16.7% compared to 0.42%–4.9% for the conventional system. Both systems showed good linearity in the concentration range of 0.8– 6.38 mM for the glass device and 5–130 ?M for the conventional system. The relationship between temperature and high voltage with baseline drift was also investigated. These results provide a foundation for the development of a miniaturised chemical analysis system for the on-line analysis of phenols in water.

Full text not available from this repository.

More information

Published date: August 2000
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 155783
URI: http://eprints.soton.ac.uk/id/eprint/155783
ISSN: 1432-1130
PURE UUID: 9f9f13c0-6532-4455-82e8-2206563026d5

Catalogue record

Date deposited: 15 Jul 2010 14:20
Last modified: 16 Jul 2019 23:57

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×