Individually addressable microelectrode array for monitoring oxygen and nitric oxide release

Patel, Bhavik Anil, Arundell, Martin, Quek, Ruben G.W., Harvey, Severin L.R., Ellis, Ian R., Florence, Margaret M., Cass, Anthony E.G., Schor, Ana M. and O'Hare, Danny (2008) Individually addressable microelectrode array for monitoring oxygen and nitric oxide release Analytical and Bioanalytical Chemistry, 390, (5), pp. 1379-1387. (doi:10.1007/s00216-007-1803-0).


Full text not available from this repository.


We have fabricated a six individual addressable gold working electrode microarray. The device is wirebonded to an eight-pin DIL package that can be easily interconnected to an external multi-channel potentiostat. A polyion complex film coating on the electrode surface provides a suitable coating for the growth of cells. The responses of oxygen and nitric oxide were assessed on uncoated and coated devices using electroanalytical methods. The film coating reduced the diffusion current by approximately 20% in both cases. No changes in the electrochemical mechanism were observed. Simultaneous recordings were obtained for 2 h in the presence of the cells, thus the device is stable for the duration of the bioanalytical measurements. Measurements were conducted to study the simultaneous changes in oxygen and nitric oxide levels in cultured fibroblast cells in the presence of growth hormones that cause cell proliferation. Increases in oxygen consumption of the cells were coupled with increases in nitric oxide levels when in the presence of the growth hormones. Use of a biological detergent to cause an oxidative burst resulted in a large increase in the current for potentials set to detect nitric oxide and oxygen

Item Type: Article
Digital Object Identifier (DOI): doi:10.1007/s00216-007-1803-0
ISSNs: 1618-2642 (print)
Keywords: microelectrode array, nitric oxide, oxygen, fibroblast cell, film coating
ePrint ID: 155785
Date :
Date Event
January 2008Published
Date Deposited: 28 May 2010 13:36
Last Modified: 18 Apr 2017 04:03
Further Information:Google Scholar

Actions (login required)

View Item View Item