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The role of FLO11 in saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system

The role of FLO11 in saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system
The role of FLO11 in saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system
A role of the FLO11 in Saccharomyces cerevisiae biofilm development in a flow cell system was examined. We carried out an ectopic FLO11 expression in the wild type (wt) BY4741 strain that has low levels of endogenous FLO11 transcript. In contrast to the nonadhesive wt, the FLO11 overexpression strain (BY4741 FLO11(+)) readily adhered to both liquid-hydrophobic and liquid-hydrophilic solid interfaces and was able to grow as a biofilm monolayer in a flow system. Cellular features associated with FLO11 were examined and found to be consistent with the previous studies conducted in different strains of S. cerevisiae. When grown in suspended liquid culture, BY4741 FLO11(+) formed larger cellular aggregates (clumps), consisting of from five to 60 cells, and displayed an increased cell surface hydrophobicity, without changes in the cell size or growth rate, compared to wt. However, the invasive growth associated with FLO11 expression was not observed in BY4741 FLO11(+). The significance of these findings is discussed in the context of clinically and industrially relevant biofilms.
biofilm adhesion, biofilm development, invasive growth, cell aggregation, FLO11, saccharomyces cerevisiae
1567-1356
372-379
Purevdorj-Gage, Boloroo
52409d1c-5d25-44e5-b552-b6cbff92965c
Orr, Miranda E.
dfa87209-3600-423f-8ca9-d8bc2347ae37
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Sheehan, Kathy B.
e2f936fe-ee72-446f-b7af-171ccc47ae5a
Hyman, Linda E.
7b266f6f-5eb4-405a-8155-d2cbdaaf37ac
Purevdorj-Gage, Boloroo
52409d1c-5d25-44e5-b552-b6cbff92965c
Orr, Miranda E.
dfa87209-3600-423f-8ca9-d8bc2347ae37
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Sheehan, Kathy B.
e2f936fe-ee72-446f-b7af-171ccc47ae5a
Hyman, Linda E.
7b266f6f-5eb4-405a-8155-d2cbdaaf37ac

Purevdorj-Gage, Boloroo, Orr, Miranda E., Stoodley, Paul, Sheehan, Kathy B. and Hyman, Linda E. (2007) The role of FLO11 in saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system. FEMS Yeast Research, 7 (3), 372-379. (doi:10.1111/j.1567-1364.2006.00189.x).

Record type: Article

Abstract

A role of the FLO11 in Saccharomyces cerevisiae biofilm development in a flow cell system was examined. We carried out an ectopic FLO11 expression in the wild type (wt) BY4741 strain that has low levels of endogenous FLO11 transcript. In contrast to the nonadhesive wt, the FLO11 overexpression strain (BY4741 FLO11(+)) readily adhered to both liquid-hydrophobic and liquid-hydrophilic solid interfaces and was able to grow as a biofilm monolayer in a flow system. Cellular features associated with FLO11 were examined and found to be consistent with the previous studies conducted in different strains of S. cerevisiae. When grown in suspended liquid culture, BY4741 FLO11(+) formed larger cellular aggregates (clumps), consisting of from five to 60 cells, and displayed an increased cell surface hydrophobicity, without changes in the cell size or growth rate, compared to wt. However, the invasive growth associated with FLO11 expression was not observed in BY4741 FLO11(+). The significance of these findings is discussed in the context of clinically and industrially relevant biofilms.

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More information

Published date: 19 January 2007
Keywords: biofilm adhesion, biofilm development, invasive growth, cell aggregation, FLO11, saccharomyces cerevisiae
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 155939
URI: http://eprints.soton.ac.uk/id/eprint/155939
ISSN: 1567-1356
PURE UUID: 1b1ab49c-e600-45cf-a4a0-e58a12f1c0f2
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

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Date deposited: 08 Jun 2010 08:58
Last modified: 14 Mar 2024 02:55

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Contributors

Author: Boloroo Purevdorj-Gage
Author: Miranda E. Orr
Author: Paul Stoodley ORCID iD
Author: Kathy B. Sheehan
Author: Linda E. Hyman

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