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Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP

Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP
Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP
Staphylococcus aureus and Staphylococcus epidermidis associated with implantable medical devices, are often difficult to treat with conventional antimicrobials. Formation of a biofilm and subsequent production of toxins are two distinct mechanisms considered important in foreign body infections. Staphylococcal virulence is caused by a complex regulatory process, which involves cell-to-cell communication through the release and response to chemical signals in a process known as quorum sensing. We explored the possibility of preventing infections by interfering with biofilm formation and toxin production using the quorum sensing inhibitor ribonucleic-acid-III-inhibiting peptide. In our studies ribonucleic-acid-III-inhibiting peptide prevented graft-associated infections caused by all species of staphylococci tested so far, including methicillin resistant S. aureus and S. epidermidis. Ribonucleic-acid-III-inhibiting peptide also enhances the effects of antibiotics and cationic peptides in the clearance of normally recalcitrant biofilm infections. Ribonucleic-acid-III-inhibiting peptide is nontoxic, highly stable, and no resistant strains have been found so far, suggesting that ribonucleic-acid-III-inhibiting peptide may be used to coat medical devices or used systemically to prevent infections. When the target of ribonucleic-acid-III activating protein activity is disrupted, biofilm formation is reduced under flow and static conditions and genes important for toxin production or biofilm formation are down-regulated. These in vitro data help explain why ribonucleic-acid-III-inhibiting peptide seems to be effective in preventing staphylococcal infections.
0009-921X
48-54
Balaban, Naomi
a171574f-cbbe-469e-afbd-b0ad346f9a45
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Fux, Christoph A
b961b84d-c1c1-4cb1-b118-a691d8de55c5
Wilson, Suzanne
7e50f2dc-d19f-41a4-b951-72e080f0fc4e
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Dell'Acqua, Giorgio
1f39d7c2-10aa-4ffd-9d91-49349141612d
Balaban, Naomi
a171574f-cbbe-469e-afbd-b0ad346f9a45
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Fux, Christoph A
b961b84d-c1c1-4cb1-b118-a691d8de55c5
Wilson, Suzanne
7e50f2dc-d19f-41a4-b951-72e080f0fc4e
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Dell'Acqua, Giorgio
1f39d7c2-10aa-4ffd-9d91-49349141612d

Balaban, Naomi, Stoodley, Paul, Fux, Christoph A, Wilson, Suzanne, Costerton, J. William and Dell'Acqua, Giorgio (2005) Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP. Clinical Orthopaedics and Related Research, (437), 48-54.

Record type: Article

Abstract

Staphylococcus aureus and Staphylococcus epidermidis associated with implantable medical devices, are often difficult to treat with conventional antimicrobials. Formation of a biofilm and subsequent production of toxins are two distinct mechanisms considered important in foreign body infections. Staphylococcal virulence is caused by a complex regulatory process, which involves cell-to-cell communication through the release and response to chemical signals in a process known as quorum sensing. We explored the possibility of preventing infections by interfering with biofilm formation and toxin production using the quorum sensing inhibitor ribonucleic-acid-III-inhibiting peptide. In our studies ribonucleic-acid-III-inhibiting peptide prevented graft-associated infections caused by all species of staphylococci tested so far, including methicillin resistant S. aureus and S. epidermidis. Ribonucleic-acid-III-inhibiting peptide also enhances the effects of antibiotics and cationic peptides in the clearance of normally recalcitrant biofilm infections. Ribonucleic-acid-III-inhibiting peptide is nontoxic, highly stable, and no resistant strains have been found so far, suggesting that ribonucleic-acid-III-inhibiting peptide may be used to coat medical devices or used systemically to prevent infections. When the target of ribonucleic-acid-III activating protein activity is disrupted, biofilm formation is reduced under flow and static conditions and genes important for toxin production or biofilm formation are down-regulated. These in vitro data help explain why ribonucleic-acid-III-inhibiting peptide seems to be effective in preventing staphylococcal infections.

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More information

Published date: August 2005
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 155959
URI: https://eprints.soton.ac.uk/id/eprint/155959
ISSN: 0009-921X
PURE UUID: 96ab79d0-b8b5-4cef-8b5a-329547e96e7f

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Date deposited: 08 Jun 2010 14:05
Last modified: 18 Jul 2017 12:43

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Contributors

Author: Naomi Balaban
Author: Paul Stoodley
Author: Christoph A Fux
Author: Suzanne Wilson
Author: J. William Costerton
Author: Giorgio Dell'Acqua

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