Can laboratory reference strains mirror "real-world" pathogenesis?
Can laboratory reference strains mirror "real-world" pathogenesis?
The extraordinary plasticity of bacterial genomes raises concerns about the adequacy of laboratory-adapted reference strains for the study of "real-world" pathogenesis. Some laboratory strains have been sub-cultured for decades since their first isolation and might have lost important pathophysiological characteristics. Evidence is presented that bacteria rapidly adapt to in vitro conditions. Genomic differences between laboratory reference strains and corresponding low-passage clinical isolates are reviewed. It appears that no bacterial strain can truly represent its species. For DNA microarray and proteomic studies, this limitation might be overcome by the summation of individual genomes to produce a species-specific virtual supragenome.
58-63
Fux, C.A.
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Shirtliff, M.
702d81d8-f4c5-48b2-900a-a69a0e892861
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Costerton, J.W.
1be42ff0-b76b-47e5-83a7-67bd2905dfc4
February 2005
Fux, C.A.
d5e858ec-fff4-485f-a894-04dfaf6fb4fe
Shirtliff, M.
702d81d8-f4c5-48b2-900a-a69a0e892861
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Costerton, J.W.
1be42ff0-b76b-47e5-83a7-67bd2905dfc4
Fux, C.A., Shirtliff, M., Stoodley, P. and Costerton, J.W.
(2005)
Can laboratory reference strains mirror "real-world" pathogenesis?
Trends in Microbiology, 13 (2), .
(doi:10.1016/j.tim.2004.11.001).
Abstract
The extraordinary plasticity of bacterial genomes raises concerns about the adequacy of laboratory-adapted reference strains for the study of "real-world" pathogenesis. Some laboratory strains have been sub-cultured for decades since their first isolation and might have lost important pathophysiological characteristics. Evidence is presented that bacteria rapidly adapt to in vitro conditions. Genomic differences between laboratory reference strains and corresponding low-passage clinical isolates are reviewed. It appears that no bacterial strain can truly represent its species. For DNA microarray and proteomic studies, this limitation might be overcome by the summation of individual genomes to produce a species-specific virtual supragenome.
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Published date: February 2005
Organisations:
Engineering Mats & Surface Engineerg Gp
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Local EPrints ID: 155975
URI: http://eprints.soton.ac.uk/id/eprint/155975
ISSN: 0966-842X
PURE UUID: cac855d5-20e1-475f-897b-377a7a038ae5
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Date deposited: 08 Jun 2010 16:13
Last modified: 14 Mar 2024 02:55
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Author:
C.A. Fux
Author:
M. Shirtliff
Author:
J.W. Costerton
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