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Toxin profile, pigment composition, and large subunit RDNA phylogenetic analysis of an Alexandrium minutum (Dinophyceae) strain isolated from the Fleet Lagoon, United Kingdom

Nascimento, S.M., Purdie, D.A., Lilly, E.L., Larsen, J. and Morris, S. (2005) Toxin profile, pigment composition, and large subunit RDNA phylogenetic analysis of an Alexandrium minutum (Dinophyceae) strain isolated from the Fleet Lagoon, United Kingdom Journal of Phycology, 41, (2), pp. 343-353. (doi:10.1111/j.1529-8817.2005.03088.x).

Record type: Article

Abstract

Paralytic shellfish toxins, pigment composition, and large subunit (LSU) rDNA sequence were analyzed for a clonal culture of Alexandrium minutum Halim isolated in 2000 from the coastal Fleet Lagoon, Dorset, United Kingdom. The HPLC pigment analysis revealed the presence of chl a, peridinin, and diadinoxanthin as major pigments and chl c1+c2 and c3, diatoxanthin, and -carotene as minor components. The toxins responsible for paralytic shellfish poisoning were analyzed by HPLC with postcolumn derivatization and fluorescence detection. The paralytic shellfish poisoning toxin profile of the Fleet Lagoon strain of A. minutum in exponential growth phase was dominated by gonyautoxin-3 up to 54%, whereas gonyautoxin-2 made up 10% and saxitoxin (STX) 36%. The average toxicity of the culture was 3.8 pg STX Eq·cell1, and total toxin content varied from 5.6 fmol·cell1 on day 1 to a maximum of 16.8 fmol·cell1 during the early stationary phase. Sequence analysis of the LSU rDNA revealed the strain to be closely related to several European strains of A. minutum and one isolated from Australian waters, although most of these do not produce STX. The shallow Fleet Lagoon may provide a favorable environment for A. minutum to bloom, and the presence of highly potent saxitoxins in this strain indicates potential for future shellfish contamination.

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More information

Published date: April 2005
Keywords: alexandrium minutum, coastal lagoon, large subunit (LSU) ribosomal DNA, paralytic shellfish poisoning, pigments, saxitoxin
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 15646
URI: http://eprints.soton.ac.uk/id/eprint/15646
ISSN: 0022-3646
PURE UUID: 0ba9a256-08ac-439f-bcf8-1354c6135fb9

Catalogue record

Date deposited: 23 May 2005
Last modified: 17 Jul 2017 16:48

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Contributors

Author: S.M. Nascimento
Author: D.A. Purdie
Author: E.L. Lilly
Author: J. Larsen
Author: S. Morris

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