The University of Southampton
University of Southampton Institutional Repository

Acylation of streptomyces type II polyketide synthase acyl carrier proteins

Crosby, John, Byrom, Kate J., Hitchman, Timothy S., Cox, Russell J., Crump, Matthew P., Findlow, I. Stuart C., Bibb, Maureen J. and Simpson, Thomas J. (1998) Acylation of streptomyces type II polyketide synthase acyl carrier proteins FEBS Letters, 433, (1-2), pp. 132-138.

Record type: Article

Abstract

Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type II FAS ACPs were made.

PDF login?target=http:%2F%2Fdevel.eprints.soton.ac.uk%2F147747%2F1%2F1998_acylation_of_Strep_type_II_PKS_ACPs.pdf - Version of Record
Restricted to Registered users only
Download (7kB)

More information

Published date: 14 August 1998

Identifiers

Local EPrints ID: 156641
URI: http://eprints.soton.ac.uk/id/eprint/156641
ISSN: 0014-5793
PURE UUID: 180fbf8a-0e54-405c-9a3d-5b5ce84c3296

Catalogue record

Date deposited: 01 Jun 2010 12:21
Last modified: 01 Oct 2017 10:54

Export record

Contributors

Author: John Crosby
Author: Kate J. Byrom
Author: Timothy S. Hitchman
Author: Russell J. Cox
Author: Matthew P. Crump
Author: I. Stuart C. Findlow
Author: Maureen J. Bibb
Author: Thomas J. Simpson

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×