The University of Southampton
University of Southampton Institutional Repository

The malonyl transferase activity of type II polyketide synthase acyl carrier proteins

Arthur, Christopher J., Szafranska, Anna E., Long, Jed, Mills, Jane, Cox, Russell J., Findlow, Stuart C., Simpson, Thomas J., Crump, Matthew P. and Crosby, John (2006) The malonyl transferase activity of type II polyketide synthase acyl carrier proteins Chemistry & Biology, 13, (6), p. 587.

Record type: Article

Abstract

Acyl carrier proteins (ACPs) play a fundamental role in directing intermediates among the enzyme active sites of fatty acid and polyketide synthases (PKSs). In this paper, we demonstrate that the Streptomyces coelicolor (S. coelicolor) actinorhodin (act) PKS ACP can catalyze transfer of malonate to type II S. coelicolor fatty acid synthase (FAS) and other PKS ACPs in vitro. The reciprocal transfer from S. coelicolor FAS ACP to a PKS ACP was not observed. Several mutations in both act ACP and S. coelicolor FAS ACP could be classified by their participation in either donation or acceptance of this malonyl group. These mutations indicated that self-malonylation and malonyl transfer could be completely decoupled, implying that they were separate processes and that a FAS ACP could be converted from a non-malonyl-transferring protein to one with malonyl transferase activity.

PDF login?target=http:%2F%2Fdevel.eprints.soton.ac.uk%2F147759%2F1%2F2006_Arthur_The_malonyl_transferase_activity_of_type_II_PKS_ACPs.pdf - Other
Restricted to Registered users only
Download (7kB)

More information

Published date: June 2006

Identifiers

Local EPrints ID: 156701
URI: http://eprints.soton.ac.uk/id/eprint/156701
ISSN: 1074-5521
PURE UUID: 0b7ab9e6-0270-4e43-a193-14e89c1c0b56

Catalogue record

Date deposited: 01 Jun 2010 13:55
Last modified: 13 Oct 2017 11:43

Export record

Contributors

Author: Christopher J. Arthur
Author: Anna E. Szafranska
Author: Jed Long
Author: Jane Mills
Author: Russell J. Cox
Author: Stuart C. Findlow
Author: Thomas J. Simpson
Author: Matthew P. Crump
Author: John Crosby

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×