Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing

Adams, Heather, Winston, Matthew T., Heersink, Joanna, Buckingham-Meyer, Kelli A., Costerton, J. William and Stoodley, Paul (2002) Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing. [in special issue: Special Issue] American Journal of Dentistry, 15, 12B-17B.

Abstract

PURPOSE: To develop an interproximal laboratory model to compare the potential effectiveness of powered brushing to remove biofilm plaque from interproximal spaces beyond the reach of bristles. MATERIALS AND METHODS: Streptococcus mutans biofilms were first grown on glass microscope slides in a drip-flow reactor. The slides were removed and positioned in the interproximal model. Each slide was exposed to 15 seconds powered brushing with either the Sonicare Elite or the Braun Oral-B 3D Excel. The thickness of the biofilm was measured with confocal microscopy at various distances from the bristle tips. RESULTS: The Sonicare Elite reduced the thickness of biofilm by 57% at a distance of 0-5 mm from the bristle tips, 53% at 5-10 mm and 43% at 10-15 mm, relative to biofilm in areas unexposed to brushing. All reductions in thickness were statistically significant (P < 0.01). The Braun Oral-B 3D Excel reduced the biofilm thickness by 16%, 13%, and 19% at the same distances respectively, but the thickness reductions were not statistically significant from those in the unexposed areas (P > 0.1).

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Contributors

Author: Paul Stoodley

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