The University of Southampton
University of Southampton Institutional Repository

The influence of hydrogen bubble formation on the removal of Pseudomonas fluorescens biofilms from platinum electrode surfaces

The influence of hydrogen bubble formation on the removal of Pseudomonas fluorescens biofilms from platinum electrode surfaces
The influence of hydrogen bubble formation on the removal of Pseudomonas fluorescens biofilms from platinum electrode surfaces
Hydrogen bubble formation on the surface of platinum electrodes as a means of removing biofilms was studied. Biofilms of Pseudomonas fluorescens of different ages were grown on platinum electrodes and challenged with hydrogen bubbles formed at the surface of the electrodes, by cycling the potential at ?2.0 V. The removal of the biofilms from the surfaces was assessed by direct epifluorescence microscopy. The removal of the biofilm from the surface was dependent on the biofilm age. As the biofilm became older, the duration of bubble formation needed to achieve complete removal changed, but in some cases, it was not possible to obtain a completely clean surface. An enhancement of biofilm removal was obtained if the potential was cycled between ?0.5 and 1.0 V for 30 min prior to bubble generation, probably due to the weakness of the forces established between the surface and the biofilm and within the biofilm.

pseudomonas fluorescens, biofilms, cyclic voltammetry, hydrogen bubbles, detachment
1359-5113
1815-1821
Gião, Maria Salomé
5638b770-3681-48b2-a9ae-9152b36ac504
Montenegro, Maria Irene
967a627e-5b8f-4ba1-b9c1-9ae5ce175697
Vieira, Maria João
68560235-8ce6-4721-b780-242508ec557c
Gião, Maria Salomé
5638b770-3681-48b2-a9ae-9152b36ac504
Montenegro, Maria Irene
967a627e-5b8f-4ba1-b9c1-9ae5ce175697
Vieira, Maria João
68560235-8ce6-4721-b780-242508ec557c

Gião, Maria Salomé, Montenegro, Maria Irene and Vieira, Maria João (2005) The influence of hydrogen bubble formation on the removal of Pseudomonas fluorescens biofilms from platinum electrode surfaces. Process Biochemistry, 40 (5), 1815-1821. (doi:10.1016/j.procbio.2004.06.068).

Record type: Article

Abstract

Hydrogen bubble formation on the surface of platinum electrodes as a means of removing biofilms was studied. Biofilms of Pseudomonas fluorescens of different ages were grown on platinum electrodes and challenged with hydrogen bubbles formed at the surface of the electrodes, by cycling the potential at ?2.0 V. The removal of the biofilms from the surfaces was assessed by direct epifluorescence microscopy. The removal of the biofilm from the surface was dependent on the biofilm age. As the biofilm became older, the duration of bubble formation needed to achieve complete removal changed, but in some cases, it was not possible to obtain a completely clean surface. An enhancement of biofilm removal was obtained if the potential was cycled between ?0.5 and 1.0 V for 30 min prior to bubble generation, probably due to the weakness of the forces established between the surface and the biofilm and within the biofilm.

Text
Giao_et_al_2005.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

Published date: 2005
Keywords: pseudomonas fluorescens, biofilms, cyclic voltammetry, hydrogen bubbles, detachment

Identifiers

Local EPrints ID: 157155
URI: https://eprints.soton.ac.uk/id/eprint/157155
ISSN: 1359-5113
PURE UUID: 0bea7f38-38f5-4cae-ac4f-56cdba7f0dcb

Catalogue record

Date deposited: 30 Jun 2010 13:24
Last modified: 19 Jul 2019 23:19

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×