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Monitoring biofilm formation by using cyclic voltammetry – effect of the experimental conditions on biofilm removal and activity

Monitoring biofilm formation by using cyclic voltammetry – effect of the experimental conditions on biofilm removal and activity
Monitoring biofilm formation by using cyclic voltammetry – effect of the experimental conditions on biofilm removal and activity
The effect of experimental conditions on cyclic voltammetry experiments on platinum electrodes
covered with biofilms formed by Pseudomonas fluorescens for 2 hours was investigated. Results show that
recycling the potential stabilizes the shape of the cyclic voltammogram after 135 cycles, but the observation
of the electrodes by epifluorescence microscopy showed that cells are still adhered to the platinum surface.
Some experimental conditions were changed during the electrochemical measurements – sweep rate, pH of
the buffer and applied potential range. Some of these parameters had a strong impact on the bacteria that
are adhered to the surface, increasing the death and removal in some circumstances.
0273-1223
51-56
Giao, M. S.
5638b770-3681-48b2-a9ae-9152b36ac504
Montenegro, M I.
504876ab-6d79-46e7-8ff7-9434dc578f95
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Giao, M. S.
5638b770-3681-48b2-a9ae-9152b36ac504
Montenegro, M I.
504876ab-6d79-46e7-8ff7-9434dc578f95
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577

Giao, M. S., Montenegro, M I. and Vieira, M.J. (2003) Monitoring biofilm formation by using cyclic voltammetry – effect of the experimental conditions on biofilm removal and activity. Water Science & Technology, 47 (5), 51-56.

Record type: Article

Abstract

The effect of experimental conditions on cyclic voltammetry experiments on platinum electrodes
covered with biofilms formed by Pseudomonas fluorescens for 2 hours was investigated. Results show that
recycling the potential stabilizes the shape of the cyclic voltammogram after 135 cycles, but the observation
of the electrodes by epifluorescence microscopy showed that cells are still adhered to the platinum surface.
Some experimental conditions were changed during the electrochemical measurements – sweep rate, pH of
the buffer and applied potential range. Some of these parameters had a strong impact on the bacteria that
are adhered to the surface, increasing the death and removal in some circumstances.

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Published date: 2003

Identifiers

Local EPrints ID: 157161
URI: http://eprints.soton.ac.uk/id/eprint/157161
ISSN: 0273-1223
PURE UUID: 06cefc6f-311b-42e6-8077-3f19037baa57

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Date deposited: 09 Jul 2010 10:55
Last modified: 14 Mar 2024 01:46

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Contributors

Author: M. S. Giao
Author: M I. Montenegro
Author: M.J. Vieira

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