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Visualization and characterization of dynamic patterns of flow, growth and activity of Vibrio fischeri biofilms growing in porous media

Sharp, R.R., Stoodley, Paul, Adgie, M., Gerlach, R. and Cunningham, A. (2005) Visualization and characterization of dynamic patterns of flow, growth and activity of Vibrio fischeri biofilms growing in porous media Water Science & Technology, 52, (7), pp. 85-90.

Record type: Article


Using a mesoscale porous media flat plate reactor we utilized a naturally bioluminescent biofilm (V. fischeri) and dye studies to obtain valuable information on the interactions between biofilms and reactive flow in porous media. The growth and development of the V. fischeri biofilm in a porous media geometry was studied using digital time lapse images of the bioluminescent signal given off by the developing biofilm. The effect of biofilm development on porous media hydrodynamics was examined using dye tracer studies and image analysis. The natural bioluminescence of the V. fischeri allowed real-time, in-situ study of biofilm development in porous media, without destruction of the biofilm. Dye studies and image analysis enabled the study of effects of biofilm accumulation on porous media hydraulics, with comparisons to plug flow and completely mixed systems with varying degrees of biofilm accumulation. The real-time nature of the study permitted us to visualize dynamic flow channel formation within the biofilm/porous media system. In addition, the sensitivity of the V. fischeri biofilm to dissolved oxygen allowed us to capture real-time images of reactive transport within the system. This work is the first meso-scale visualization of the interactions between biofilm and flow in porous media.

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Published date: 2005
Organisations: Engineering Mats & Surface Engineerg Gp


Local EPrints ID: 157163
ISSN: 0273-1223
PURE UUID: ba6d1ed5-5afe-4965-8460-935fc6c25147

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Date deposited: 08 Jun 2010 16:40
Last modified: 18 Jul 2017 12:41

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Author: R.R. Sharp
Author: Paul Stoodley
Author: M. Adgie
Author: R. Gerlach
Author: A. Cunningham

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