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Influence of hydrodynamics and nutrients on biofilm structure

Record type: Article

Hydrodynamic conditions control two interlinked parameters; mass transfer and drag, and will, therefore, significantly influence many of the processes involved in biofilm development. The goal of this research was to determine the effect of flow velocity and nutrients on biofilm structure. Biofilms were grown in square glass capillary flow cells under laminar and turbulent flows. Biofilms were observed microscopically under flow conditions using image analysis. Mixed species bacterial biofilms were grown with glucose (40 mg/l) as the limiting nutrient. Biofilms grown under laminar conditions were patchy and consisted of roughly circular cell clusters separated by interstitial voids. Biofilms in the turbulent flow cell were also patchy but these biofilms consisted of patches of ripples and elongated 'streamers' which oscillated in the flow. To assess the influence of changing nutrient conditions on biofilm structure the glucose concentration was increased from 40 to 400 mg/l on an established 21 day old biofilm growing in turbulent flow. The cell clusters grew rapidly and the thickness of the biofilm increased from 30 µm to 130 µm within 17 h. The ripples disappeared after 10 hours. After 5 d the glucose concentration was reduced back to 40 mg/l. There was a loss of biomass and patches of ripples were re-established within a further 2 d.

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Citation

Stoodley, P., Dodds, I., Boyle, J.D. and Lappin-Scott, H.M. (1999) Influence of hydrodynamics and nutrients on biofilm structure Journal of Applied Microbiology, 85, 19S-28S. (doi:10.1111/j.1365-2672.1998.tb05279.x).

More information

Published date: 1999
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 157179
URI: http://eprints.soton.ac.uk/id/eprint/157179
ISSN: 1364-5072
PURE UUID: 465985a1-18bf-45a7-b9af-3b045003f988

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Date deposited: 15 Jun 2010 09:05
Last modified: 18 Jul 2017 12:41

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Contributors

Author: P. Stoodley
Author: I. Dodds
Author: J.D. Boyle
Author: H.M. Lappin-Scott

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