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Growth and detachment of cell clusters from mature mixed-species biofilms

Stoodley, P., Wilson, S., Hall-Stoodley, L., Boyle, J.D., Lappin-Scott, H.M. and Costerton, J.W. (2001) Growth and detachment of cell clusters from mature mixed-species biofilms Applied and Environmental Microbiology, 67, (12), pp. 5608-5613. (doi:10.1128/AEM.67.12.5608-5613.2001.).

Record type: Article

Abstract

Detachment from biofilms is an important consideration in the dissemination of infection and the contamination of industrial systems but is the least-studied biofilm process. By using digital time-lapse microscopy and biofilm flow cells, we visualized localized growth and detachment of discrete cell clusters in mature mixed-species biofilms growing under steady conditions in turbulent flow in situ. The detaching biomass ranged from single cells to an aggregate with a diameter of approximately 500 microm. Direct evidence of local cell cluster detachment from the biofilms was supported by microscopic examination of filtered effluent. Single cells and small clusters detached more frequently, but larger aggregates contained a disproportionately high fraction of total detached biomass. These results have significance in the establishment of an infectious dose and public health risk assessment.

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More information

Published date: December 2001
Organisations: Engineering Mats & Surface Engineerg Gp

Identifiers

Local EPrints ID: 157451
URI: http://eprints.soton.ac.uk/id/eprint/157451
ISSN: 0099-2240
PURE UUID: d73de098-cfa0-462b-83ef-85f23f5971b7

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Date deposited: 09 Jun 2010 15:40
Last modified: 18 Jul 2017 12:40

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Contributors

Author: P. Stoodley
Author: S. Wilson
Author: L. Hall-Stoodley
Author: J.D. Boyle
Author: H.M. Lappin-Scott
Author: J.W. Costerton

University divisions

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