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Recognition of auto- and exoantigens by V4-34 gene encoded antibodies

Recognition of auto- and exoantigens by V4-34 gene encoded antibodies
Recognition of auto- and exoantigens by V4-34 gene encoded antibodies
The antigenic specificities of 24 V4-34-encoded monoclonal antibodies were compared with the amino acid sequence. The specificities were divided into three categories, red blood cells, B lymphocytes and auto/exoantigens. Six anti-I monoclonal antibodies, with multiple substitutions in their VH region, did not bind B lymphocytes or auto/exoantigens. Reactivity to these two antigens segregated with the 16 anti-i monoclonal antibodies, which were derived from the near germline V4-34 gene. All anti-i monoclonal antibodies bound B lymphocytes, albeit with varying intensities. B-cell binding correlated with basic amino acids in the VH-CDR3. Reactivity to auto/exoantigens was demonstrated only by a subset anti-i monoclonal antibodies and did not correlate with B-lymphocyte or i-antigen binding. These anti-ssDNA reactive monoclonal antibodies had basic amino acids in the VH-CDR3, strongly supporting the suggested role of arginine in DNA binding. However, an arginine-rich CDR3 was not enough to ensure DNA reactivity, since six other anti-i monoclonal antibodies that fulfilled this criteria did not bind ssDNA. Thus it is possible that the anti-DNA reactivity of V4-34-encoded monoclonal antibodies is mediated by the classic antigen-binding groove generated by the CDRs of the heavy/light chains. In contrast, anti-B-cell/i-antigen reactivity is mediated, unconventionally, by the V4-34 protein with a dominant influence of the VH-CDR3.
0300-9475
134-140
Bhat, N.M.
41aa3f51-0846-46d2-828d-d29392534d4d
Bieber, M.M.
ec926e74-9d2a-4538-84d4-6bdc4f4eab52
Spellerberg, M.B.
f68fa135-0aef-4be2-826f-9ed21d18785b
Stevenson, F.K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Teng, N.N.
1300e3f2-53a1-49c5-b588-85b817d8de31
Bhat, N.M.
41aa3f51-0846-46d2-828d-d29392534d4d
Bieber, M.M.
ec926e74-9d2a-4538-84d4-6bdc4f4eab52
Spellerberg, M.B.
f68fa135-0aef-4be2-826f-9ed21d18785b
Stevenson, F.K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Teng, N.N.
1300e3f2-53a1-49c5-b588-85b817d8de31

Bhat, N.M., Bieber, M.M., Spellerberg, M.B., Stevenson, F.K. and Teng, N.N. (2000) Recognition of auto- and exoantigens by V4-34 gene encoded antibodies. Scandinavian Journal of Immunology, 51 (2), 134-140.

Record type: Article

Abstract

The antigenic specificities of 24 V4-34-encoded monoclonal antibodies were compared with the amino acid sequence. The specificities were divided into three categories, red blood cells, B lymphocytes and auto/exoantigens. Six anti-I monoclonal antibodies, with multiple substitutions in their VH region, did not bind B lymphocytes or auto/exoantigens. Reactivity to these two antigens segregated with the 16 anti-i monoclonal antibodies, which were derived from the near germline V4-34 gene. All anti-i monoclonal antibodies bound B lymphocytes, albeit with varying intensities. B-cell binding correlated with basic amino acids in the VH-CDR3. Reactivity to auto/exoantigens was demonstrated only by a subset anti-i monoclonal antibodies and did not correlate with B-lymphocyte or i-antigen binding. These anti-ssDNA reactive monoclonal antibodies had basic amino acids in the VH-CDR3, strongly supporting the suggested role of arginine in DNA binding. However, an arginine-rich CDR3 was not enough to ensure DNA reactivity, since six other anti-i monoclonal antibodies that fulfilled this criteria did not bind ssDNA. Thus it is possible that the anti-DNA reactivity of V4-34-encoded monoclonal antibodies is mediated by the classic antigen-binding groove generated by the CDRs of the heavy/light chains. In contrast, anti-B-cell/i-antigen reactivity is mediated, unconventionally, by the V4-34 protein with a dominant influence of the VH-CDR3.

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Published date: February 2000

Identifiers

Local EPrints ID: 157807
URI: https://eprints.soton.ac.uk/id/eprint/157807
ISSN: 0300-9475
PURE UUID: 47f65c96-5dee-4160-b078-691daae39d85
ORCID for F.K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

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Date deposited: 06 Jul 2010 16:01
Last modified: 06 Jun 2018 13:01

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Contributors

Author: N.M. Bhat
Author: M.M. Bieber
Author: M.B. Spellerberg
Author: F.K. Stevenson ORCID iD
Author: N.N. Teng

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